Human Talin-1, TLN1 ELISA Kit | MyBioSource MBS904386 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Talin-1, TLN1 ELISA Kit

catalog :

MBS904386

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS904386

products type :

ELISA Kit

products full name :

Human Talin-1, TLN1 ELISA Kit

products short name :

talin 1

products name syn :

Human Talin-1 (TLN1) ELISA kit; ILWEQ; KIAA1027; TLN; ; talin 1

other names :

talin-1; Talin-1; talin-1; talin 1

products gene name :

TLN1

other gene names :

TLN1; TLN1; TLN; ILWEQ; KIAA1027; TLN

uniprot entry name :

TLN1_HUMAN

reactivity :

Human

sequence length :

2541

specificity :

This assay has high sensitivity and excellent specificity for detection of human TLN1. No significant cross-reactivity or interference between human TLN1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 23.5 pg/ml -1500 pg/ml. Sensitivity: 5.8 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TLN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TLN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

223029410

ncbi acc num :

NP_006280.3

ncbi gb acc num :

NM_006289.3

uniprot acc num :

Q9Y490

ncbi mol weight :

269,767 Da

ncbi pathways :

Activation Of Chaperone Genes By XBP1(S) Pathway (530771); Activation Of Chaperones By IRE1alpha Pathway (105906); Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); Diabetes Pathways (105902); Disease Pathway (530764); EGF Receptor (ErbB1) Signaling Pathway (137970); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478)

ncbi summary :

This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]

uniprot summary :

Talin-1: a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. Protein type: Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 9p13. Cellular Component: ruffle; cell surface; focal adhesion; cytoplasm; plasma membrane; extracellular region; microtubule organizing center; intercellular junction; cytosol. Molecular Function: actin filament binding; integrin binding; LIM domain binding; protein binding; structural constituent of cytoskeleton; insulin receptor binding; vinculin binding. Biological Process: platelet activation; axon guidance; intercellular junction assembly; unfolded protein response; cytoskeletal anchoring; cellular protein metabolic process; platelet degranulation; unfolded protein response, activation of signaling protein activity; muscle contraction; cell-substrate junction assembly; cortical actin cytoskeleton organization and biogenesis; cell motility; blood coagulation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage