catalog number :
MBS904375
products type :
ELISA Kit
products full name :
Dog Granulocyte colony-stimulating factor, CSF3 ELISA Kit
products short name :
colony stimulating factor 3 (granulocyte)
products name syn :
Dog Granulocyte colony-stimulating factor (CSF3) ELISA kit; G-CSF; GCSF; MGC45931; colony stimulating factor 3; filgrastim; granulocyte colony stimulating factor; lenograstim; pluripoietin; colony stimulating factor 3 (granulocyte)
other names :
Granulocyte colony-stimulating factor; Granulocyte colony-stimulating factor
products gene name :
CSF3
other gene names :
CSF3; G-CSF
uniprot entry name :
CSF3_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of Dog CSF3. No significant cross-reactivity or interference between Dog CSF3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 12.5 pg/ml-800 pg/ml. Sensitivity: 3.12 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CSF3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CSF3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CSF3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CSF3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
18,858 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
size4 :
10x96-Strip-Wells