ELISA/assay

Human Cubilin, CUBN ELISA Kit

MBS904353

48-Strip-Wells

510 USD

ELISA Kit

Human Cubilin, CUBN ELISA Kit

cubilin (intrinsic factor-cobalamin receptor)

Human Cubilin (CUBN) ELISA kit; RP11-416D8.1; FLJ90055; FLJ90747; IFCR; MGA1; gp280; cubilin; intrinsic factor B12-receptor; intrinsic factor-cobalamin receptor; intrinsic factor-vitamin B12 receptor; cubilin (intrinsic factor-cobalamin receptor)

cubilin; Cubilin; cubilin; 460 kDa receptor; cubilin precursor variant 1; cubilin precursor variant 2; intestinal intrinsic factor receptor; intrinsic factor-vitamin B12 receptor; cubilin (intrinsic factor-cobalamin receptor); 460 kDa receptor; Intestinal intrinsic factor receptor; Intrinsic factor-cobalamin receptor; Intrinsic factor-vitamin B12 receptor

CUBN

CUBN; CUBN; IFCR; MGA1; gp280; IFCR

CUBN_HUMAN

Human

3623

This assay has high sensitivity and excellent specificity for detection of Human CUBN. No significant cross-reactivity or interference between Human CUBN and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 9.38 pg/ml-600 pg/ml. Sensitivity: 2.35 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Lipogenesis and lipometabolism. Protein Biological Process 3: Cholesterol metabolism

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CUBN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CUBN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CUBN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CUBN bound in the initial step. The color development is stopped and the intensity of the color is measured.

126091152

NP_001072.2

NM_001081.3

O60494

398,736 Da

HDL-mediated Lipid Transport Pathway (106158); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamins A And D Pathway (106150); Vitamin D (calciferol) Metabolism Pathway (160956); Vitamin Digestion And Absorption Pathway (199556); Vitamin Digestion And Absorption Pathway (199538)

Cubilin (CUBN) acts as a receptor for intrinsic factor-vitamin B12 complexes. The role of receptor is supported by the presence of 27 CUB domains. Cubulin is located within the epithelium of intestine and kidney. Mutations in CUBN may play a role in autosomal recessive megaloblastic anemia. [provided by RefSeq, Jul 2008]

CUBN: Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. Defects in CUBN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1); also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected. Chromosomal Location of Human Ortholog: 10p12.31. Cellular Component: Golgi apparatus; lysosomal lumen; lysosomal membrane; endoplasmic reticulum; brush border membrane; coated pit; cytosol; extrinsic to external side of plasma membrane; membrane; endocytic vesicle; apical plasma membrane; plasma membrane; endosome membrane. Molecular Function: protein binding; protein homodimerization activity; transporter activity; receptor activity; calcium ion binding; cobalamin binding. Biological Process: steroid metabolic process; receptor-mediated endocytosis; cholesterol metabolic process; vitamin metabolic process; cobalamin metabolic process; lipoprotein transport; cobalamin transport; tissue homeostasis; lipoprotein metabolic process; water-soluble vitamin metabolic process; vitamin D metabolic process. Disease: Megaloblastic Anemia 1

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800