ELISA/assay

Rat Runt-related transcription factor 2, RUNX2 ELISA Kit

MBS904266

48-Strip-Wells

510 USD

ELISA Kit

Rat Runt-related transcription factor 2, RUNX2 ELISA Kit

runt-related transcription factor 2

Rat Runt-related transcription factor 2 (RUNX2) ELISA kit; RP1-166H4.1; AML3; CBFA1; CCD; CCD1; MGC120022; MGC120023; OSF2; PEA2aA; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1; CBF-alpha 1; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A; runt-related transcription factor 2

Runt-related transcription factor 2; Runt-related transcription factor 2; runt-related transcription factor 2; CBF-alpha-1; core-binding factor subunit alpha-1; runt related transcription factor 2; osteoblast-specific transcription factor 2; runt-related transcription factor 2; Core-binding factor subunit alpha-1; CBF-alpha-1; Osteoblast-specific transcription factor 2; OSF-2

RUNX2

Runx2; Runx2; Cbfa1; OSF-2; Cbfa1; Osf2; CBF-alpha-1; OSF-2

RUNX2_RAT

Rat

218

This assay has high sensitivity and excellent specificity for detection of rat RUNX2. No significant cross-reactivity or interference between rat RUNX2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml-200 ng/ml. Sensitivity: The minimum detectable dose of rat RUNX2 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RUNX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RUNX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RUNX2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RUNX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

21362908

Q9Z2J9.2

Q9Z2J9

24,247 Da

Androgen Receptor Signaling Pathway (198529); Endochondral Ossification Pathway (219771); TGF Beta Signaling Pathway (198444); TGF-beta Receptor Signaling Pathway (198439); Transcriptional Misregulation In Cancer Pathway (523027); Transcriptional Misregulation In Cancer Pathway (522987); Wnt Signaling Pathway NetPath (198488)

AML3: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5 - PYGPYGGT-3 , of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors. Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 and HIPK3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with KAT6A and KAT6B. Binds to cyclin B1 CCNB1. Interacts with DDX5. Specifically expressed in osteoblasts. 3 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Transcription factor. Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nuclear chromatin; nucleus. Molecular Function: protein domain specific binding; DNA binding; histone deacetylase binding; bHLH transcription factor binding; chromatin binding; transcription factor binding; transcription factor activity; ATP binding. Biological Process: embryonic forelimb morphogenesis; transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; cell maturation; BMP signaling pathway; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; chondrocyte differentiation; skeletal development; regulation of odontogenesis of dentine-containing teeth; T cell differentiation; endochondral ossification; transcription, DNA-dependent; regulation of osteoblast differentiation; regulation of fibroblast growth factor receptor signaling pathway; chondrocyte development; stem cell differentiation; embryonic cranial skeleton morphogenesis; positive regulation of ossification; odontogenesis of dentine-containing teeth; osteoblast development; osteoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of chondrocyte differentiation; skeletal morphogenesis; negative regulation of smoothened signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of ossification; osteoblast fate commitment

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800