Human Betaine--homocysteine S-methyltransferase 1, BHMT ELISA Kit | MyBioSource MBS904226 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Betaine--homocysteine S-methyltransferase 1, BHMT ELISA Kit

catalog :

MBS904226

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS904226

products type :

ELISA Kit

products full name :

Human Betaine--homocysteine S-methyltransferase 1, BHMT ELISA Kit

products short name :

betaine-homocysteine methyltransferase

products name syn :

Human Betaine--homocysteine S-methyltransferase 1 (BHMT) ELISA kit; betaine homocysteine methyltransferase; betaine-homocysteine methyltransferase

other names :

betaine--homocysteine S-methyltransferase 1; Betaine--homocysteine S-methyltransferase 1; betaine--homocysteine S-methyltransferase 1; epididymis secretory sperm binding protein Li 61p; betaine--homocysteine S-methyltransferase

products gene name :

BHMT

other gene names :

BHMT; BHMT; BHMT1; HEL-S-61p

uniprot entry name :

BHMT1_HUMAN

reactivity :

Human

sequence length :

406

specificity :

This assay has high sensitivity and excellent specificity for detection of human BHMT. No significant cross-reactivity or interference between human BHMT and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human BHMT is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BHMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BHMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BHMT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BHMT bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

157266337

ncbi acc num :

NP_001704.2

ncbi gb acc num :

NM_001713.2

uniprot acc num :

Q93088

ncbi mol weight :

44,998 Da

ncbi pathways :

Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421); Glycine, Serine And Threonine Metabolism Pathway (82949); Glycine, Serine And Threonine Metabolism Pathway (313); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); One Carbon Metabolism Pathway (198756); Sulfur Amino Acid Metabolism Pathway (530753); Glycine Betaine Degradation Pathway (142322); Methionine Salvage II (mammalia) Pathway (142159)

ncbi summary :

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]

uniprot summary :

BHMT: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. Protein type: Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - glycine, serine and threonine; Methyltransferase; EC 2.1.1.5. Chromosomal Location of Human Ortholog: 5q14.1. Cellular Component: protein complex; cytoplasm; cytosol. Molecular Function: betaine-homocysteine S-methyltransferase activity; homocysteine S-methyltransferase activity; zinc ion binding; protein complex binding. Biological Process: S-methylmethionine cycle; protein amino acid methylation; betaine catabolic process; sulfur amino acid metabolic process; S-adenosylmethionine metabolic process; response to organic nitrogen; regulation of homocysteine metabolic process; betaine metabolic process

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage