catalog number :
MBS904155
products type :
ELISA Kit
products full name :
Mouse neuromedin U, NMU ELISA Kit
products short name :
neuromedin U
products name syn :
Mouse neuromedin U (NMU) ELISA kit; neuromedin U
other names :
neuromedin-U; Neuromedin-U; neuromedin-U; neuromedin U
other gene names :
Nmu; Nmu; NmU-23
uniprot entry name :
NMU_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse NMU. No significant cross-reactivity or interference between Mouse NMU and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.9 ng/ml-60 ng/ml. Sensitivity: 0.225 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NMU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NMU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NMU is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NMU bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_062388.1
ncbi gb acc num :
NM_019515.1
ncbi mol weight :
19,429 Da
ncbi pathways :
G Alpha (i) Signalling Events Pathway (575454); G Alpha (q) Signalling Events Pathway (575456); GPCR Downstream Signaling Pathway (575452); Signal Transduction Pathway (575318); Signaling By GPCR Pathway (575424)
uniprot summary :
NMU: Stimulates muscle contractions of specific regions of the gastrointestinal tract. In humans, NmU stimulates contractions of the ileum and urinary bladder. Belongs to the NmU family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular region; terminal button. Molecular Function: neuromedin U receptor binding. Biological Process: regulation of smooth muscle contraction; gastric acid secretion; neuropeptide signaling pathway; eating behavior; positive regulation of hormone secretion; positive regulation of smooth muscle contraction; sensory perception of pain; positive regulation of synaptic transmission
size4 :
10x96-Strip-Wells