ELISA/assay

Human Kunitz-type protease inhibitor 1, SPINT1 ELISA Kit

MBS904050

48-Strip-Wells

510 USD

ELISA Kit

Human Kunitz-type protease inhibitor 1, SPINT1 ELISA Kit

serine peptidase inhibitor, Kunitz type 1

Human Kunitz-type protease inhibitor 1 (SPINT1) ELISA kit; HAI; HAI1; MANSC2; Kunitz-type protease inhibitor 1; hepatocyte growth factor activator inhibitor 1; serine protease inhibitor; Kunitz type 1; serine peptidase inhibitor; Kunitz type 1

kunitz-type protease inhibitor 1 isoform 2; Kunitz-type protease inhibitor 1; kunitz-type protease inhibitor 1; HAI-1; serine protease inhibitor, Kunitz type 1; hepatocyte growth factor activator inhibitor type 1; serine peptidase inhibitor, Kunitz type 1; Hepatocyte growth factor activator inhibitor type 1; HAI-1

SPINT1

SPINT1; SPINT1; HAI; HAI1; MANSC2; HAI1; UNQ223/PRO256; HAI-1

SPIT1_HUMAN

Human

513

This assay has high sensitivity and excellent specificity for detection of human SPINT1. No significant cross-reactivity or interference between human SPINT1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0. 156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human SPINT1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPINT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPINT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPINT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPINT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

74027265

NP_001027539.1

NM_001032367.1

O43278

58,398 Da

Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]

SPINT1: Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14). 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 15q15.1. Cellular Component: extracellular space; membrane; extracellular region. Molecular Function: serine-type endopeptidase inhibitor activity. Biological Process: extracellular matrix organization and biogenesis

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800