ELISA/assay

Human Glutathione S-transferase Mu 1, GSTM1 ELISA Kit

MBS903572

48-Strip-Wells

510 USD

ELISA Kit

Human Glutathione S-transferase Mu 1, GSTM1 ELISA Kit

glutathione S-transferase mu 1

Human Glutathione S-transferase Mu 1 (GSTM1) ELISA kit; GST1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; H-B; MGC26563; MU; MU-1; GST class-mu 1; HB subunit 4; S- (hydroxyalkyl) glutathione lyase; glutathione S-alkyltransferase; glutathione S-aralkyltransferas; glutathione S-transferase mu 1

glutathione S-transferase Mu 1 isoform 1; Glutathione S-transferase Mu 1; glutathione S-transferase Mu 1; HB subunit 4; GST class-mu 1; GST HB subunit 4; glutathione S-transferase M1; glutathione S-aryltransferase; glutathione S-alkyltransferase; glutathione S-aralkyltransferase; S-(hydroxyalkyl)glutathione lyase; glutathione S-transferase mu 1; GST HB subunit 4; GST class-mu 1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4

GSTM1

GSTM1; GSTM1; MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GST1

GSTM1_HUMAN

Human

218

This assay has high sensitivity and excellent specificity for detection of human GSTM1. No significant cross-reactivity or interference between human GSTM1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.47 mU/ml -30 mU/ml. Sensitivity: The minimum detectable dose of human GSTM1 is typically less than 0.11 mU/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GSTM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GSTM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GSTM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GSTM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

23065544

NP_000552.2

NM_000561.3

P09488

25,712 Da

Biological Oxidations Pathway (105698); C-MYB Transcription Factor Network Pathway (138073); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343); Metabolism Pathway (477135); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (83031)

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008]

GSTM1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Mu family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 2.5.1.18. Chromosomal Location of Human Ortholog: 1p13.3. Cellular Component: cytoplasm; cytosol. Molecular Function: protein homodimerization activity; enzyme binding; glutathione transferase activity; glutathione binding. Biological Process: glutathione metabolic process; nitrobenzene metabolic process; xenobiotic metabolic process; xenobiotic catabolic process

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800