Mouse Peroxisome Proliferator-activated Receptor beta, PPAR-beta ELISA Kit | MyBioSource MBS903532 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Peroxisome Proliferator-activated Receptor beta, PPAR-beta ELISA Kit

catalog :

MBS903532

quantity :

48-Strip-Wells

price :

565 USD

product information

catalog number :

MBS903532

products type :

ELISA Kit

products full name :

Mouse Peroxisome Proliferator-activated Receptor beta, PPAR-beta ELISA Kit

products short name :

Peroxisome Proliferator-activated Receptor beta (PPAR-beta)

products name syn :

Mouse Peroxisome Proliferator-activated Receptor beta (PPAR-beta) ELISA Kit; FAAR; MGC3931; NR1C2; NUC1; NUCI; NUCII; PPAR-beta; PPARB; OTTHUMP00000016256; OTTHUMP00000016257; nuclear hormone receptor 1; peroxisome proliferative activated receptor; delta; Peroxisome Proliferator-activated Receptor beta (PPAR-beta)

other names :

peroxisome proliferator-activated receptor delta; Peroxisome proliferator-activated receptor delta; peroxisome proliferator-activated receptor delta; PPARdelta/beta; nuclear hormone receptor 1; nuclear receptor subfamily 1 group C member 2; Peroxisome proliferator-activated receptor beta; peroxisome proliferator activator receptor delta; Nuclear hormone receptor 1; NUC1; Nuclear receptor subfamily 1 group C member 2; Peroxisome proliferator-activated receptor beta; PPAR-beta

products gene name :

PPAR-beta

other gene names :

Ppard; Ppard; NUC1; NUC-1; Nr1c2; Pparb; PPAR[b]; Pparb/d; PPAR-beta; PPARdelta; PPAR-delta; Nr1c2; Pparb; PPAR-delta; NUC1; PPAR-beta

uniprot entry name :

PPARD_MOUSE

reactivity :

Mouse

sequence length :

440

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse PPAR-beta. No significant cross-reactivity or interference between mouse PPAR-beta and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: The minimum detectable dose of mouse PPAR-beta is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PPAR-beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPAR-beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPAR-beta is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPAR-beta bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

33859590

ncbi acc num :

NP_035275.1

ncbi gb acc num :

NM_011145.3

uniprot acc num :

P35396

ncbi mol weight :

49,715 Da

ncbi pathways :

Acute Myeloid Leukemia Pathway (83310); Acute Myeloid Leukemia Pathway (529); Adipogenesis Pathway (198299); Gene Expression Pathway (574654); Generic Transcription Pathway (574655); Nuclear Receptor Transcription Pathway (574657); Nuclear Receptors Pathway (198318); Nuclear Receptors In Lipid Metabolism And Toxicity Pathway (198356); PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway (522983); PPAR Signaling Pathway (83239)

uniprot summary :

PPAR-delta: Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. Belongs to the nuclear hormone receptor family. NR1 subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Nuclear receptor; DNA-binding. Cellular Component: nucleus. Molecular Function: ligand-dependent nuclear receptor activity; zinc ion binding; prostacyclin receptor activity; metal ion binding; transcription coactivator activity; enzyme activator activity; drug binding; retinoid X receptor binding; NF-kappaB binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; steroid hormone receptor activity; fatty acid binding; lipid binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; positive regulation of catalytic activity; proteoglycan metabolic process; epidermis development; wound healing; negative regulation of smooth muscle cell proliferation; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; negative regulation of collagen biosynthetic process; positive regulation of epidermis development; negative regulation of smooth muscle cell migration; negative regulation of transcription from RNA polymerase II promoter; positive regulation of vasodilation; vitamin A metabolic process; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; axon ensheathment; cell differentiation; negative regulation of epithelial cell proliferation; placenta development; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of insulin secretion; keratinocyte proliferation; keratinocyte migration; cell-substrate adhesion; regulation of cell proliferation; phospholipid biosynthetic process; positive regulation of phosphoinositide 3-kinase cascade; regulation of transcription from RNA polymerase II promoter; cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of inflammatory response; mRNA transcription; fatty acid oxidation; steroid hormone mediated signaling; negative regulation of cell growth; lipid metabolic process; anagen; cellular process; regulation of insulin secretion; embryo implantation; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150