Mouse Claudin-5, CLDN5 ELISA Kit | MyBioSource MBS903469 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Claudin-5, CLDN5 ELISA Kit

catalog :

MBS903469

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS903469

products type :

ELISA Kit

products full name :

Mouse Claudin-5, CLDN5 ELISA Kit

products short name :

claudin 5

products name syn :

Mouse Claudin-5 (CLDN5) ELISA kit; AWAL; BEC1; CPETRL1; TMVCF; OTTHUMP00000197372; OTTHUMP00000197819; androgen withdrawal and apoptosis induced protein RVP1-like; transmembrane protein deleted in velocardiofacial syndrome; claudin 5

other names :

claudin-5; Claudin-5; claudin-5; lung-specific membrane protein; transmembrane protein deleted in VCFS; brain endothelial cell clone 1 protein; claudin 5; Brain endothelial cell clone 1 protein; Lung-specific membrane protein

products gene name :

CLDN5

other gene names :

Cldn5; Cldn5; MBEC1; Tmvcf; AI854493; Bec1

uniprot entry name :

CLD5_MOUSE

reactivity :

Mouse

sequence length :

218

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse CLDN5. No significant cross-reactivity or interference between mouse CLDN5 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml -1200 pg/ml. Sensitivity: The minimum detectable dose of mouse CLDN5 is typically less than 4.68 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CLDN5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLDN5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLDN5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLDN5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

31980735

ncbi acc num :

NP_038833.2

ncbi gb acc num :

NM_013805.4

uniprot acc num :

O54942

ncbi mol weight :

23,054 Da

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Circadian Exercise Pathway (198298); Diurnally Regulated Genes With Circadian Orthologs Pathway (198406); Hepatitis C Pathway (173974); Hepatitis C Pathway (173907); Leukocyte Transendothelial Migration Pathway (83280); Leukocyte Transendothelial Migration Pathway (494); Tight Junction Pathway (83268); Tight Junction Pathway (482)

ncbi summary :

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a critical component of endothelial tight junctions that control pericellular permeability. The knockout mice lacking this gene died within 10 h of birth and the blood-brain barrier in these mice against small molecules was selectively affected. This gene is expressed strongly in endothelium of normal lung and plays a regulation role during acrolein-induced acute lung injury. [provided by RefSeq, Aug 2010]

uniprot summary :

Claudin-5: Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family. Protein type: Membrane protein, multi-pass; Cytoskeletal; Membrane protein, integral. Cellular Component: tight junction; membrane; paranode region of axon; plasma membrane; integral to membrane; intercellular junction; cell junction. Molecular Function: protein binding; structural molecule activity. Biological Process: myelination; cell-cell adhesion

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage