ELISA/assay

Mouse Protein Wnt-4, WNT4 ELISA Kit

MBS903402

48-Strip-Wells

510 USD

ELISA Kit

Mouse Protein Wnt-4, WNT4 ELISA Kit

wingless-type MMTV integration site family, member 4

Mouse Protein Wnt-4 (WNT4) ELISA kit; RP1-224A6.7; SERKAL; WNT-4; WNT-4 protein; wingless-type MMTV integration site family; member 4

protein Wnt-4; Protein Wnt-4; protein Wnt-4; signal molecule; wingless-related MMTV integration site 4; wingless-type MMTV integration site family, member 4

WNT4

Wnt4; Wnt4; Wnt-4; Wnt-4

WNT4_MOUSE

Mouse

351

This assay has high sensitivity and excellent specificity for detection of mouse WNT4. No significant cross-reactivity or interference between mouse WNT4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: The minimum detectable dose of mouse WNT4 is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6678595

NP_033549.1

NM_009523.2

P22724

39,050 Da

Basal Cell Carcinoma Pathway (83306); Basal Cell Carcinoma Pathway (525); ESC Pluripotency Pathways (198374); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83260); Hedgehog Signaling Pathway (474); Melanogenesis Pathway (83289); Melanogenesis Pathway (504); Pathways In Cancer (83298)

WNT4: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Overexpression may be associated with abnormal proliferation in human breast tissue. Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome); also called Mayer- Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5 000 newborn girls. Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL); also known as SERKAL syndrome. Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL). Belongs to the Wnt family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; cell surface; cytoplasm; extracellular region. Molecular Function: protein binding; frizzled binding; transcription corepressor activity; receptor binding. Biological Process: anatomical structure development; negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of collagen biosynthetic process; T cell differentiation in the thymus; thyroid stimulating hormone secreting cell differentiation; androgen biosynthetic process; Wnt receptor signaling pathway through beta-catenin; signal transduction; somatotropin secreting cell differentiation; neuron differentiation; regulation of cell-cell adhesion; positive regulation of focal adhesion formation; cell-cell signaling; positive regulation of aldosterone biosynthetic process; positive regulation of stress fiber formation; embryonic epithelial tube formation; mesonephros development; cell differentiation; negative regulation of cell migration; female gonad development; cell fate commitment; Wnt receptor signaling pathway; positive regulation of meiosis; male gonad development; immature T cell proliferation in the thymus; cellular response to starvation; positive regulation of bone mineralization; positive regulation of osteoblast differentiation; negative regulation of cell differentiation; protein palmitoylation; branching morphogenesis of a tube; ureteric bud branching; gamete generation; hormone metabolic process; smooth muscle cell differentiation; female sex determination; tube morphogenesis; negative regulation of transcription, DNA-dependent; sex differentiation; metanephros development; oocyte development; positive regulation of GTPase activity

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800