catalog number :
MBS903372
products type :
ELISA Kit
products full name :
Rat Neuropilin-2, NRP2 ELISA Kit
products short name :
neuropilin 2
products name syn :
Rat Neuropilin-2 (NRP2) ELISA kit; MGC126574; NP2; NPN2; PRO2714; VEGF165R2; OTTHUMP00000206355; neuropilin-2a (17) ; neuropilin-2a (22) ; neuropilin-2b (0) ; receptor for VEGF165 and semaphorins class3; vascular endothelial growth factor-165 r; neuropilin 2
other names :
neuropilin-2; Neuropilin-2; neuropilin-2; vascular endothelial cell growth factor 165 receptor 2; neuropilin 2; Vascular endothelial cell growth factor 165 receptor 2
products gene name :
NRP2
other gene names :
Nrp2; Nrp2
uniprot entry name :
NRP2_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat NRP2. No significant cross-reactivity or interference between rat NRP2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: Typically less than 0.078 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NRP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NRP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRP2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_110496.1
ncbi gb acc num :
NM_030869.3
ncbi mol weight :
103,897 Da
ncbi pathways :
Axon Guidance Pathway (574273); Developmental Biology Pathway (574272); L1CAM Interactions Pathway (574293); Neurophilin Interactions With VEGF And VEGFR Pathway (574198); NrCAM Interactions Pathway (574298); Signal Transduction Pathway (574116); Signaling By VEGF Pathway (574195); VEGF Ligand-receptor Interactions Pathway (574196)
ncbi summary :
an axonal guidance molecule [RGD, Feb 2006]
uniprot summary :
Function: High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.
size4 :
10x96-Strip-Wells