Human Occludin, OCLN ELISA Kit | MyBioSource MBS903313 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Occludin, OCLN ELISA Kit

catalog :

MBS903313

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS903313

products type :

ELISA Kit

products full name :

Human Occludin, OCLN ELISA Kit

products short name :

[occludin]

products name syn :

[Human Occludin (OCLN) ELISA kit; tight junction protein occludin TM4 minus; occludin]

other names :

[occludin isoform a; Occludin; occludin; tight junction protein occludin; phosphatase 1, regulatory subunit 115; occludin]

products gene name :

[OCLN]

other gene names :

[OCLN; OCLN; BLCPMG; PPP1R115]

uniprot entry name :

OCLN_HUMAN

reactivity :

Human

sequence length :

522

specificity :

This assay has high sensitivity and excellent specificity for detection of human OBP2A. No significant cross-reactivity or interference between human OBP2A and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OBP2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OBP2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OBP2A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OBP2A bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

327478414

ncbi acc num :

NP_001192183.1

ncbi gb acc num :

NM_001205254.1

uniprot acc num :

Q16625

ncbi mol weight :

59,144 Da

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); Pathogenic Escherichia Coli Infection Pathway (83103); Pathogenic Escherichia Coli Infection Pathway (516); TGF-beta Receptor Signaling Pathway (138024); Tight Junction Pathway (83071)

ncbi summary :

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]

uniprot summary :

occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral. Chromosomal Location of Human Ortholog: 5q13.1. Cellular Component: tight junction; endocytic vesicle; apicolateral plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; intercellular junction; cytoplasmic vesicle; cytosol; cell junction. Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity. Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process; protein complex assembly; cell structure disassembly during apoptosis. Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145