catalog number :
MBS903309
products type :
ELISA Kit
products full name :
Rat Myelin-oligodendrocyte glycoprotein, MOG ELISA Kit
products short name :
myelin oligodendrocyte glycoprotein
products name syn :
Rat Myelin-oligodendrocyte glycoprotein (MOG) ELISA kit; DAQB-92E24.2; MGC26137; MOGIG2; MOG Ig-AluB; myelin oligodendrocyte glycoprotein
other names :
Myelin-oligodendrocyte glycoprotein; Myelin-oligodendrocyte glycoprotein
uniprot entry name :
MOG_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat MOG. No significant cross-reactivity or interference between rat MOG and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of rat MOG is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MOG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MOG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MOG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MOG bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
27,882 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Mediates homophilic cell-cell adhesion Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.
size4 :
10x96-Strip-Wells