ELISA/assay

Mouse Adenosine receptor A2b, ADORA2B ELISA Kit

MBS903160

48-Strip-Wells

510 USD

ELISA Kit

Mouse Adenosine receptor A2b, ADORA2B ELISA Kit

adenosine A2b receptor

Mouse Adenosine receptor A2b (ADORA2B) ELISA kit; ADORA2; ; adenosine A2b receptor

adenosine receptor A2b; Adenosine receptor A2b; adenosine receptor A2b; A2b, Rs; adenosine A2b receptor

ADORA2B

Adora2b; Adora2b; A2b; A2BR; A2BAR; AA2BR; AI480866; AI605384

AA2BR_MOUSE

Mouse

332

This assay has high sensitivity and excellent specificity for detection of Mouse ADORA2B. No significant cross-reactivity or interference between Mouse ADORA2B and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.81 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADORA2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADORA2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADORA2B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADORA2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

31560741

NP_031439.2

NM_007413.4

Q60614

36,120 Da

Adenosine P1 Receptors Pathway (575445); Alcoholism Pathway (585577); Alcoholism Pathway (587116); Calcium Signaling Pathway (83247); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (575426); G Alpha (s) Signalling Events Pathway (575453); GPCR Downstream Signaling Pathway (575452); GPCR Ligand Binding Pathway (575425); GPCRs, Class A Rhodopsin-like Pathway (198308)

ADORA2B: Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family. Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass. Cellular Component: cell surface; membrane; integral to membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; signal transducer activity; adenosine receptor activity, G-protein coupled. Biological Process: relaxation of vascular smooth muscle; negative regulation of collagen biosynthetic process; positive regulation of interleukin-6 production; positive regulation of guanylate cyclase activity; positive regulation of mast cell degranulation; positive regulation of vasodilation; signal transduction; G-protein signaling, adenylate cyclase activating pathway; regulation of angiogenesis; positive regulation of chemokine production; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; positive regulation of cGMP biosynthetic process; positive regulation of chronic inflammatory response to non-antigenic stimulus; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; cellular response to extracellular stimulus; positive regulation of catecholamine secretion; adenosine receptor signaling pathway

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800