Human Histidyl-tRNA synthetase, cytoplasmic, HARS ELISA Kit | MyBioSource MBS902901 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Histidyl-tRNA synthetase, cytoplasmic, HARS ELISA Kit

catalog :

MBS902901

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS902901

products type :

ELISA Kit

products full name :

Human Histidyl-tRNA synthetase, cytoplasmic, HARS ELISA Kit

products short name :

histidyl-tRNA synthetase

products name syn :

Human Histidyl-tRNA synthetase; cytoplasmic (HARS) ELISA kit; FLJ20491; HRS; HisRS; histidine tRNA ligase 1; cytoplasmic; histidine translase; histidine-tRNA ligase; histidyl-tRNA synthetase

other names :

histidine--tRNA ligase, cytoplasmic isoform 2; Histidine--tRNA ligase, cytoplasmic; histidine--tRNA ligase, cytoplasmic; HisRS; histidine translase; histidyl-tRNA synthetase; Histidyl-tRNA synthetase; HisRS

products gene name :

HARS

other gene names :

HARS; HARS; HRS; USH3B; HRS; HisRS

uniprot entry name :

SYHC_HUMAN

reactivity :

Human

sequence length :

469

specificity :

This assay has high sensitivity and excellent specificity for detection of human HARS. No significant cross-reactivity or interference between human HARS and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human HARS is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HARS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HARS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HARS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HARS bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

384475552

ncbi acc num :

NP_001244969.1

ncbi gb acc num :

NM_001258040.2

uniprot acc num :

P12081

ncbi mol weight :

57,411 Da

ncbi pathways :

Aminoacyl-tRNA Biosynthesis Pathway (83030); Aminoacyl-tRNA Biosynthesis Pathway (424); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (413439); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (468360); Cytosolic TRNA Aminoacylation Pathway (105981); Gene Expression Pathway (105937); TRNA Aminoacylation Pathway (105980)

ncbi summary :

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

uniprot summary :

HARS: Defects in HARS are a cause of Usher syndrome type 3B (USH3B). USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called Charles Bonnet syndrome, involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life. Belongs to the class-II aminoacyl-tRNA synthetase family. Protein type: EC 6.1.1.21; Translation; Ligase. Chromosomal Location of Human Ortholog: 5q31.3. Cellular Component: cytoplasm; cytosol. Molecular Function: histidine-tRNA ligase activity; ATP binding. Biological Process: tRNA aminoacylation for protein translation; translation; gene expression; histidyl-tRNA aminoacylation. Disease: Usher Syndrome, Type Iiib

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage