catalog number :
MBS902741
products type :
ELISA Kit
products full name :
Mouse WAP four-disulfide core domain protein 2, WFDC2 ELISA Kit
products short name :
WAP four-disulfide core domain 2
products name syn :
Mouse WAP four-disulfide core domain protein 2 (WFDC2) ELISA kit; EDDM4; HE4; MGC57529; WAP5; dJ461P17.6; WAP domain containing protein HE4-V4; epididymal protein 4; epididymal secretory protein E4; epididymis-specific; whey-acidic protein type; four-disulfide core; m; WAP four-disulfide core domain 2
other names :
WAP four-disulfide core domain protein 2; WAP four-disulfide core domain protein 2; WAP four-disulfide core domain protein 2; WAP domain-containing protein HE4; WAP four-disulfide core domain 2; WAP domain-containing protein HE4
products gene name :
WFDC2
other gene names :
Wfdc2; Wfdc2; HE4; WAP5; 1600023A02Rik; He4
uniprot entry name :
WFDC2_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse WFDC2. No significant cross-reactivity or interference between mouse WFDC2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of mouse WFDC2 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WFDC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WFDC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WFDC2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WFDC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_080599.1
ncbi gb acc num :
NM_026323.2
ncbi mol weight :
18,032 Da
uniprot summary :
WFDC2: a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular region. Molecular Function: serine-type endopeptidase inhibitor activity; aspartic-type endopeptidase inhibitor activity; protease inhibitor activity; cysteine protease inhibitor activity. Biological Process: negative regulation of peptidase activity
size4 :
10x96-Strip-Wells