Mouse Group 10 secretory phospholipase A2, PLA2G10 ELISA Kit | MyBioSource MBS902713 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Group 10 secretory phospholipase A2, PLA2G10 ELISA Kit

catalog :

MBS902713

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS902713

products type :

ELISA Kit

products full name :

Mouse Group 10 secretory phospholipase A2, PLA2G10 ELISA Kit

products short name :

phospholipase A2, group X

products name syn :

Mouse Group 10 secretory phospholipase A2 (PLA2G10) ELISA kit; GXPLA2; GXSPLA2; MGC119918; MGC119919; MGC133367; SPLA2; ; phospholipase A2; group X

other names :

group 10 secretory phospholipase A2 isoform 1 preproprotein; Group 10 secretory phospholipase A2; group 10 secretory phospholipase A2; GX sPLA2; group X secretory phospholipase A2; phosphatidylcholine 2-acylhydrolase 10; phospholipase A2, group X; Group X secretory phospholipase A2; GX sPLA2; sPLA2-X; Phosphatidylcholine 2-acylhydrolase 10

products gene name :

PLA2G10

other gene names :

Pla2g10; Pla2g10; PLA2GX; sPLA2-X; mGXsPLA2; GX sPLA2; sPLA2-X

uniprot entry name :

PA2GX_MOUSE

reactivity :

Mouse

sequence length :

151

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse PLA2G10. No significant cross-reactivity or interference between mouse PLA2G10 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of mouse PLA2G10 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLA2G10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLA2G10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

21356649

ncbi acc num :

NP_036117.1

ncbi gb acc num :

NM_011987.3

uniprot acc num :

Q9QXX3

ncbi mol weight :

17,005 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (83190); Arachidonic Acid Metabolism Pathway (366); Ether Lipid Metabolism Pathway (83189); Ether Lipid Metabolism Pathway (365); Fat Digestion And Absorption Pathway (194386); Fat Digestion And Absorption Pathway (194324); Fc Epsilon RI Signaling Pathway (83279); Fc Epsilon RI Signaling Pathway (493); Glutamatergic Synapse Pathway (213819); Glutamatergic Synapse Pathway (213811)

ncbi summary :

This gene encodes a member of the phospholipase A2 family of lipolytic enzymes that hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids. The encoded protein undergoes proteolytic processing to generate a calcium-dependent enzyme that plays pivotal roles in the liberation of arachidonic acid from membrane phospholipids leading to the production of various inflammatory lipid mediators, such as prostaglandins. In response to myocardial ischemia/reperfusion, mice lacking the encoded protein display a reduction in myocardial infarct size partly through the suppression of neutorphil cytotoxic activities. Alternative splicing results in multiple transcript variants encoding different isoforms. All of these isoforms may undergo similar processing to generate the mature protein. [provided by RefSeq, Jul 2015]

uniprot summary :

PLA2G10: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine. Belongs to the phospholipase A2 family. Protein type: Secreted, signal peptide; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Motility/polarity/chemotaxis; Secreted; Phospholipase; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; Cell development/differentiation; EC 3.1.1.4; Lipid Metabolism - alpha-linolenic acid. Cellular Component: extracellular region. Molecular Function: phospholipase A2 activity; hydrolase activity; metal ion binding; calcium ion binding; phospholipase activity. Biological Process: axon guidance; cholesterol homeostasis; phospholipid metabolic process; lysophospholipid transport; regulation of macrophage activation; negative regulation of transcription factor activity; positive regulation of cellular protein metabolic process; positive regulation of prostaglandin secretion; lipid metabolic process; lipid catabolic process

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage