catalog number :
MBS902630
products type :
ELISA Kit
products full name :
Mouse Lymphotoxin-beta, LTB ELISA Kit
products short name :
lymphotoxin beta (TNF superfamily, member 3)
products name syn :
Mouse Lymphotoxin-beta (LTB) ELISA kit; DAAP-90L16.4; TNFC; TNFSF3; p33; lymphotoxin-beta; tumor necrosis factor C; lymphotoxin beta (TNF superfamily; member 3)
other names :
lymphotoxin-beta; Lymphotoxin-beta; lymphotoxin-beta; TNF-C; LT-beta; lymphotoxin beta; tumor necrosis factor C; tumor necrosis factor ligand superfamily member 3; lymphotoxin B; Tumor necrosis factor C; TNF-C; Tumor necrosis factor ligand superfamily member 3
other gene names :
Ltb; Ltb; p33; Tnfc; LTbeta; Tnfsf3; AI662801; Tnfc; Tnfsf3; LT-beta; TNF-C
uniprot entry name :
TNFC_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse LTB. No significant cross-reactivity or interference between mouse LTB and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 12.5 pg/ml -800 pg/ml. Sensitivity: The minimum detectable dose of mouse LTB is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LTB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LTB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LTB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LTB bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_032544.1
ncbi gb acc num :
NM_008518.2
ncbi mol weight :
32,329 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); NF-kappa B Signaling Pathway (634537); Rheumatoid Arthritis Pathway (200310); Rheumatoid Arthritis Pathway (200269)
uniprot summary :
LTB: Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface. Isoform 2 is probably non-functional. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Cellular Component: extracellular space; membrane; integral to membrane; plasma membrane. Molecular Function: cytokine activity; tumor necrosis factor receptor binding. Biological Process: skin development; positive regulation of interleukin-12 biosynthetic process; gene expression; immune response; lymph node development
size4 :
10x96-Strip-Wells