catalog number :
MBS902439
products type :
ELISA Kit
products full name :
Rat sex hormone-binding globulin, SHBG ELISA Kit
products short name :
sex hormone-binding globulin
products name syn :
Rat sex hormone-binding globulin (SHBG) ELISA kit; ABP; MGC126834; MGC138391; SBP; TEBG; androgen binding protein; sex steroid-binding protein; testis-specific androgen-binding protein; testosterone-binding beta-globulin; testosterone-estradiol-binding; sex hormone-binding globulin
other names :
sex hormone-binding globulin; Sex hormone-binding globulin; sex hormone-binding globulin; ABP; SBP; sex steroid-binding protein; sex hormone binding globulin; androgen binding protein 1 (296 AA); sex hormone-binding globulin protein; testis-specific androgen-binding protein; Sex steroid-binding protein; SBP; Testis-specific androgen-binding protein; ABP
products gene name :
SHBG
other gene names :
SHBG; SHBG; SHBG; SBP; ABP
uniprot entry name :
SHBG_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat SHBG. No significant cross-reactivity or interference between Rat SHBG and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates, cell lysates. Detection Range: 375 ng/ml-6000 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 187.5ng/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SHBG. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated SHBG. A competitive inhibition reaction is launched between SHBG (Standards or samples) and Biotin-conjugated SHBG with the pre-coated antibody specific for SHBG. The more amount of SHBG in samples, the less antibody bound by Biotin-conjugated SHBG. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of SHBG in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075839.1
ncbi gb acc num :
NM_001082370.1
ncbi mol weight :
43,154 Da
uniprot summary :
Function: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration