Human Secreted frizzled-related protein 2, SFRP2 ELISA Kit | MyBioSource MBS902287 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Secreted frizzled-related protein 2, SFRP2 ELISA Kit

catalog :

MBS902287

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS902287

products type :

ELISA Kit

products full name :

Human Secreted frizzled-related protein 2, SFRP2 ELISA Kit

products short name :

secreted frizzled-related protein 2

products name syn :

Human Secreted frizzled-related protein 2 (SFRP2) ELISA kit; FRP-2; SARP1; SDF-5; secreted apoptosis related protein 1; secreted frizzled-related protein 2

other names :

secreted frizzled-related protein 2; Secreted frizzled-related protein 2; secreted frizzled-related protein 2; SARP-1; sFRP-2; secreted apoptosis related protein 1; secreted apoptosis-related protein 1; secreted frizzled-related protein 2; Secreted apoptosis-related protein 1; SARP-1

products gene name :

SFRP2

other gene names :

SFRP2; SFRP2; FRP-2; SARP1; SDF-5; FRP2; SARP1; FKSG12; UNQ361/PRO697; FRP-2; sFRP-2; SARP-1

uniprot entry name :

SFRP2_HUMAN

reactivity :

Human

sequence length :

295

specificity :

This assay has high sensitivity and excellent specificity for detection of human SFRP2. No significant cross-reactivity or interference between human SFRP2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates, cell lysates. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human SFRP2 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFRP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

48475052

ncbi acc num :

NP_003004.1

ncbi gb acc num :

NM_003013.2

uniprot acc num :

Q96HF1

ncbi mol weight :

33,490 Da

ncbi pathways :

Wnt Signaling Pathway NetPath (198799); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471)

ncbi summary :

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq, Jul 2008]

uniprot summary :

SFRP2: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis. Belongs to the secreted frizzled-related protein (sFRP) family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q31.3. Cellular Component: extracellular matrix; extracellular space; integral to membrane. Molecular Function: integrin binding; G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; fibronectin binding; receptor agonist activity. Biological Process: convergent extension involved in axis elongation; negative regulation of Wnt receptor signaling pathway; collagen fibril organization; positive regulation of apoptosis; negative regulation of caspase activity; negative regulation of BMP signaling pathway; negative regulation of cell proliferation; negative regulation of mesodermal cell fate specification; cell-cell signaling; positive regulation of cell proliferation; negative regulation of cell migration; response to nutrient; negative regulation of epithelial cell proliferation; response to drug; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of JNK activity; male gonad development; positive regulation of cell adhesion mediated by integrin; digestive tract morphogenesis; chondrocyte development; positive regulation of cell growth; positive regulation of peptidyl-serine phosphorylation; patterning of blood vessels; positive regulation of osteoblast differentiation; positive regulation of angiogenesis; neural tube closure; cellular response to extracellular stimulus; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage