catalog number :
MBS902280
products type :
ELISA Kit
products full name :
Pig Heparin-binding growth factor 1, FGF1 ELISA Kit
products short name :
fibroblast growth factor 1 (acidic)
products name syn :
Pig Heparin-binding growth factor 1 (FGF1) ELISA kit; AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-alpha; FGFA; GLIO703; HBGF1; OTTHUMP00000066031; endothelial cell growth factor; alpha; endothelial cell growth factor; beta; heparin-binding growth factor 1; fibroblast growth factor 1 (acidic)
other names :
Fibroblast growth factor 1; Fibroblast growth factor 1; acidic fibroblast growth factor; Heparin-binding growth factor 1; Acidic fibroblast growth factor; aFGF; Endothelial cell growth factor; ECGF; Heparin-binding growth factor 1; HBGF-1
products gene name :
FGF1
other gene names :
AFGF; FGF1; Fgf1; FGF-1; FGF-1; aFGF; ECGF; HBGF-1
uniprot entry name :
FGF1_PIG
specificity :
This assay has high sensitivity and excellent specificity for detection of pig FGF1. No significant cross-reactivity or interference between pig FGF1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 12.5 pg/ml -800 pg/ml. Sensitivity: The minimum detectable dose of pig FGF1 is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
17,103 Da
ncbi pathways :
Downstream Signaling Of Activated FGFR Pathway (578645); FGFR Ligand Binding And Activation Pathway (578632); FGFR1 Ligand Binding And Activation Pathway (578633); FGFR1b Ligand Binding And Activation Pathway (578634); FGFR1c Ligand Binding And Activation Pathway (578635); FGFR2 Ligand Binding And Activation Pathway (578636); FGFR2b Ligand Binding And Activation Pathway (578637); FGFR2c Ligand Binding And Activation Pathway (578638); FGFR3 Ligand Binding And Activation Pathway (578639); FGFR3b Ligand Binding And Activation Pathway (578640)
uniprot summary :
Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro
size4 :
10x96-Strip-Wells