catalog number :
MBS902219
products type :
ELISA Kit
products full name :
Human beta-crosslaps, bCTx ELISA Kit
products short name :
beta-crosslaps (bCTx)
products name syn :
Human beta-crosslaps (bCTx) ELISA kit; beta-crosslaps (bCTx)
products gene name :
bCTx
specificity :
This assay has high sensitivity and excellent specificity for detection of human bCTx. No significant cross-reactivity or interference between human bCTx and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Competitive. Detection Range: 125 pg/ml - 2000 pg/ml. Sensitivity: The minimum detectable dose of human bCTx is typically less than 125 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human bCTx concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to bCTx. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated bCTx. A competitive inhibition reaction is launched between bCTx (Standards or samples) and Biotin-conjugated bCTx with the pre-coated antibody specific for bCTx. The more amount of bCTx in samples, the less antibody bound by Biotin-conjugated bCTx. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of bCTx in the sample. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells