catalog number :
MBS902214
products type :
ELISA Kit
products full name :
Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 3, ENPP3 ELISA Kit
products short name :
ectonucleotide pyrophosphatase/phosphodiesterase 3
products name syn :
Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (ENPP3) ELISA kit; RP5-988G15.3; B10; CD203c; NPP3; PD-IBETA; PDNP3; OTTHUMP00000017901; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) ; dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) ; gp13; ectonucleotide pyrophosphatase/phosphodiesterase 3
other names :
ectonucleotide pyrophosphatase/phosphodiesterase family member 3; Ectonucleotide pyrophosphatase/phosphodiesterase family member 3; ectonucleotide pyrophosphatase/phosphodiesterase family member 3; E-NPP 3; PD-Ibeta; phosphodiesterase I beta; phosphodiesterase I/nucleotide pyrophosphatase 3; ectonucleotide pyrophosphatase/phosphodiesterase 3; Phosphodiesterase I beta; PD-Ibeta; Phosphodiesterase I/nucleotide pyrophosphatase 3; CD_antigen: CD203cIncluding the following 2 domains:Alkaline phosphodiesterase I (EC:3.1.4.1); Nucleotide pyrophosphatase (EC:3.6.1.9); NPPase
products gene name :
ENPP3
other gene names :
Enpp3; Enpp3; CD203c; AI876438; E-NPP 3; PD-Ibeta; NPPase
uniprot entry name :
ENPP3_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse ENPP3. No significant cross-reactivity or interference between mouse ENPP3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: The minimum detectable dose of mouse ENPP3 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ENPP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ENPP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ENPP3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ENPP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_598766.2
ncbi gb acc num :
NM_134005.2
ncbi mol weight :
98,662 Da
ncbi pathways :
Nicotinate And Nicotinamide Metabolism Pathway (83212); Nicotinate And Nicotinamide Metabolism Pathway (400); Pantothenate And CoA Biosynthesis Pathway (83213); Pantothenate And CoA Biosynthesis Pathway (401); Purine Metabolism Pathway (83144); Purine Metabolism Pathway (307); Riboflavin Metabolism Pathway (83210); Riboflavin Metabolism Pathway (398); Starch And Sucrose Metabolism Pathway (83173); Starch And Sucrose Metabolism Pathway (344)
uniprot summary :
ENPP3: Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. Protein type: Phosphodiesterase; EC 3.6.1.9; Carbohydrate Metabolism - starch and sucrose; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.4.1; Motility/polarity/chemotaxis; Membrane protein, integral; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis. Cellular Component: membrane; perinuclear region of cytoplasm; extracellular region; integral to membrane. Molecular Function: nucleotide diphosphatase activity; phosphodiesterase I activity; nucleic acid binding; hydrolase activity; metal ion binding; catalytic activity; nucleoside-triphosphate diphosphatase activity; scavenger receptor activity; polysaccharide binding. Biological Process: metabolic process; nucleoside triphosphate catabolic process; immune response; phosphate metabolic process
size4 :
10x96-Strip-Wells