catalog number :
MBS901931
products type :
ELISA Kit
products full name :
Canine Interleukin 17, IL-17 ELISA Kit
products short name :
Interleukin 17 (IL-17)
products name syn :
Canine Interleukin 17 (IL-17) ELISA Kit; CTLA8; IL-17; IL-17A; IL17; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; cytotoxic T-lymphocyte-associated serine esterase 8; interleukin 17 (cytotoxic T-ly; Interleukin 17 (IL-17)
other names :
interleukin-17A; Interleukin-17A; interleukin-17A; IL-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
products gene name :
IL17
other gene names :
IL17A; IL17A; IL17; IL-17; IL-17; IL-17A
uniprot entry name :
IL17_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of canine IL-17. No significant cross-reactivity or interference between canine IL-17 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of canine IL-17 is typically less than 7.81 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-17 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001008412.1
ncbi gb acc num :
NM_001008412.2
ncbi mol weight :
17,242 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (84290); Cytokine-cytokine Receptor Interaction Pathway (460); Rheumatoid Arthritis Pathway (200308); Rheumatoid Arthritis Pathway (200269)
uniprot summary :
Function: Induces stromal cells to produce proinflammatory and hematopoietic cytokines
size4 :
10x96-Strip-Wells