catalog number :
MBS901806
products type :
ELISA Kit
products full name :
Rat Transmembrane glycoprotein NMB, GPNMB ELISA Kit
products short name :
glycoprotein (transmembrane) nmb
products name syn :
Rat Transmembrane glycoprotein NMB (GPNMB) ELISA kit; HGFIN; NMB; glycoprotein NMB; glycoprotein nmb-like protein; osteoactivin; transmembrane glycoprotein; glycoprotein (transmembrane) nmb
other names :
Transmembrane glycoprotein NMB; Transmembrane glycoprotein NMB
products gene name :
GPNMB
uniprot entry name :
GPNMB_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat GPNMB. No significant cross-reactivity or interference between rat GPNMB and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: The minimum detectable dose of rat GPNMB is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GPNMB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPNMB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPNMB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPNMB bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
63,731 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Could be a melanogenic enzyme
size4 :
10x96-Strip-Wells