ELISA/assay

Human Secretogranin-2, SCG2 ELISA Kit

MBS901663

48-Strip-Wells

510 USD

ELISA Kit

Human Secretogranin-2, SCG2 ELISA Kit

secretogranin II (chromogranin C)

Human Secretogranin-2 (SCG2) ELISA kit; CHGC; SN; SgII; Chromogranin C (secretogranin II) ; EM66; secretogranin II; secretoneurin; secretogranin II (chromogranin C)

secretogranin-2; Secretogranin-2; secretogranin-2; secretoneurin; chromogranin-C; secretogranin II; Chromogranin-C; Secretogranin II; SgII

SCG2

SCG2; SCG2; SN; CHGC; EM66; SgII; CHGC; SgII; SN

SCG2_HUMAN

Human

617

This assay has high sensitivity and excellent specificity for detection of human SCG2. No significant cross-reactivity or interference between human SCG2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 12.5 pg/ml -800 pg/ml. Sensitivity: 3.12 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SCG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SCG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCG2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

68160947

NP_003460.2

NM_003469.4

P13521

70,941 Da

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Studies in rodents suggest that the full-length protein, secretogranin II, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. The full-length protein is cleaved to produce the active peptide secretoneurin, which exerts chemotaxic effects on specific cell types, and EM66, whose function is unknown. [provided by RefSeq, Jul 2008]

SCG2: Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides. Belongs to the chromogranin/secretogranin protein family. Protein type: Cell cycle regulation; Apoptosis; Secreted; Cytokine; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2q35-q36. Cellular Component: extracellular space; secretory granule. Molecular Function: cytokine activity; chemoattractant activity. Biological Process: positive chemotaxis; negative regulation of endothelial cell proliferation; MAPKKK cascade; eosinophil chemotaxis; protein secretion; endothelial cell migration; positive regulation of endothelial cell proliferation; angiogenesis; inflammatory response; induction of positive chemotaxis

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800