ELISA/assay

Human Natural killer cell receptor 2B4, CD244 ELISA Kit

MBS901643

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Natural killer cell receptor 2B4, CD244 ELISA Kit

[CD244 molecule, natural killer cell receptor 2B4]

[Human Natural killer cell receptor 2B4 (CD244) ELISA kit; 2B4; NAIL; NKR2B4; Nmrk; SLAMF4; CD244 natural killer cell receptor 2B4; NK cell activation inducing ligand NAIL; CD244 molecule; natural killer cell receptor 2B4]

[natural killer cell receptor 2B4 isoform 2; Natural killer cell receptor 2B4; natural killer cell receptor 2B4; h2B4; SLAM family member 4; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; NK cell activation inducing ligand NAIL; signaling lymphocytic activation molecule 4; CD244 molecule, natural killer cell receptor 2B4; NK cell activation-inducing ligand; NAIL; NK cell type I receptor protein 2B4; NKR2B4; h2B4; SLAM family member 4; SLAMF4; Signaling lymphocytic activation molecule 4; CD_antigen: CD244]

[CD244]

[CD244; CD244; 2B4; NAIL; Nmrk; NKR2B4; SLAMF4; 2B4; NAIL; NKR2B4; h2B4; SLAMF4]

CD244_HUMAN

Human

370

This assay has high sensitivity and excellent specificity for detection of human CD244. No significant cross-reactivity or interference between human CD244 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Quantitative Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: 0.156 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD244 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD244 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD244 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD244 bound in the initial step. The color development is stopped and the intensity of the color is measured.

262263435

NP_001160135.1

NM_001166663.1

Q9BZW8

41,616 Da

Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); Natural Killer Cell Mediated Cytotoxicity Pathway (83079); Natural Killer Cell Mediated Cytotoxicity Pathway (490)

This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

CD244: Modulate other receptor-ligand interactions to enhance leukocyte activation. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 1q23.3. Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane. Molecular Function: protein binding; receptor activity. Biological Process: signal transduction; blood coagulation; leukocyte migration. Disease: Rheumatoid Arthritis

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145