ELISA/assay

Mouse Integrin beta-3, ITGB3 ELISA Kit

MBS901618

48-Strip-Wells

510 USD

ELISA Kit

Mouse Integrin beta-3, ITGB3 ELISA Kit

integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)

Mouse Integrin beta-3 (ITGB3) ELISA kit; CD61; GP3A; GPIIIa; integrin beta chain; beta 3; platelet glycoprotein IIIa; integrin; beta 3 (platelet glycoprotein IIIa; antigen CD61)

integrin beta-3; Integrin beta-3; integrin beta-3; GPIIIa; platelet gpIIIa; platelet membrane glycoprotein IIIa; integrin beta 3; Platelet membrane glycoprotein IIIa; GPIIIa; CD_antigen: CD61

ITGB3

Itgb3; Itgb3; CD61; GP3A; INGRB3; GPIIIa

ITB3_MOUSE

Mouse

787

This assay has high sensitivity and excellent specificity for detection of Mouse ITGB3. No significant cross-reactivity or interference between Mouse ITGB3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITGB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITGB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ITGB3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ITGB3 bound in the initial step. The color development is stopped and the intensity of the color is measured

160358856

NP_058060.2

NM_016780.2

O54890

86,738 Da

Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117303); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Dilated Cardiomyopathy Pathway (121495); Dilated Cardiomyopathy Pathway (121285); ECM-receptor Interaction Pathway (83265); ECM-receptor Interaction Pathway (479); Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); Hematopoietic Cell Lineage Pathway (83275)

ITGB3: integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral. Cellular Component: filopodium membrane; cell surface; focal adhesion; integral to membrane; cell projection; membrane; microvillus membrane; apical plasma membrane; plasma membrane; melanosome; nucleus; cell junction; integrin complex; receptor complex; external side of plasma membrane. Molecular Function: integrin binding; identical protein binding; protein binding; protease binding; vascular endothelial growth factor receptor 2 binding; extracellular matrix binding; fibronectin binding; protein disulfide isomerase activity; cell adhesion molecule binding; receptor activity; peptide binding. Biological Process: negative regulation of lipid transport; entry of virus into host cell; protein folding; positive regulation of osteoclast differentiation; positive regulation of leukocyte migration; positive regulation of smooth muscle cell proliferation; cell-matrix adhesion; multicellular organismal development; smooth muscle cell migration; negative chemotaxis; positive regulation of smooth muscle cell migration; regulation of cell migration; positive regulation of MAPKKK cascade; cell-substrate junction assembly; positive regulation of bone resorption; cell growth; cell adhesion; integrin-mediated signaling pathway; platelet activation; cell migration; negative regulation of lipoprotein metabolic process; positive regulation of cell adhesion mediated by integrin; cell projection morphogenesis; angiogenesis involved in wound healing; cell-substrate adhesion; regulation of release of sequestered calcium ion into cytosol; cellular calcium ion homeostasis; heterotypic cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; negative regulation of low-density lipoprotein receptor biosynthetic process; positive regulation of osteoblast proliferation; activation of protein kinase activity; positive regulation of endothelial cell proliferation; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of cell migration

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800