ELISA/assay

Human Protein Wnt-5a, WNT5A ELISA Kit

MBS901538

48-Strip-Wells

510 USD

ELISA Kit

Human Protein Wnt-5a, WNT5A ELISA Kit

wingless-type MMTV integration site family, member 5A

Human Protein Wnt-5a (WNT5A) ELISA kit; hWNT5A; WNT-5A protein; wingless-type MMTV integration site family; member 5A

protein Wnt-5a isoform 2; Protein Wnt-5a; protein Wnt-5a; WNT-5A protein; wingless-type MMTV integration site family, member 5A

WNT5A

WNT5A; WNT5A; hWNT5A

WNT5A_HUMAN

Human

365

This assay has high sensitivity and excellent specificity for detection of human WNT5A. No significant cross-reactivity or interference between human WNT5A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: 0.039 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT5A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT5A bound in the initial step. The color development is stopped and the intensity of the color is measured.

371502087

NP_001243034.1

NM_001256105.1

P41221

42,339 Da

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Class B/2 (Secretin Family Receptors) Pathway (106378); DNA Damage Response (only ATM Dependent) Pathway (198827); GPCR Ligand Binding Pathway (161020); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83063); Hedgehog Signaling Pathway (474); Melanogenesis Pathway (83092)

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

WNT5A: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. Interacts with PORCN. Interacts with WLS. Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3p21-p14. Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; endoplasmic reticulum lumen; Golgi lumen; extracellular region; plasma membrane. Molecular Function: protein domain specific binding; receptor tyrosine kinase-like orphan receptor binding; frizzled binding; cytokine activity; receptor agonist activity; transcription factor activity; frizzled-2 binding. Biological Process: activation of MAPK activity; positive regulation of transcription, DNA-dependent; embryonic skeletal development; negative chemotaxis; Wnt receptor signaling pathway through beta-catenin; positive regulation of interleukin-1 beta secretion; negative regulation of synaptogenesis; uterus development; protein amino acid phosphorylation; negative regulation of BMP signaling pathway; activation of NF-kappaB transcription factor; neuron differentiation; positive regulation of fibroblast proliferation; determination of anterior/posterior axis, embryo; positive regulation of mesenchymal cell proliferation; positive regulation of macrophage activation; somitogenesis; cell fate commitment; urinary bladder development; olfactory bulb interneuron development; activation of protein kinase B; vagina development; positive regulation of interleukin-6 production; negative regulation of fat cell differentiation; keratinocyte differentiation; genitalia development; Wnt receptor signaling pathway, calcium modulating pathway; positive regulation of angiogenesis; positive regulation of protein catabolic process; midgut development; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; lens development in camera-type eye; axon guidance; wound healing; hypophysis morphogenesis; negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of cytokine secretion during immune response; palate development; negative regulation of axon extension involved in axon guidance; activation of JNK activity; response to organic substance; establishment of planar polarity; heart looping; negative regulation of epithelial cell proliferation; cervix development; Wnt receptor signaling pathway; positive regulation of meiosis; male gonad development; positive regulation of peptidyl-serine phosphorylation; positive regulation of ossification; positive regulation of cGMP metabolic process; convergent extension involved in organogenesis; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; ameboidal cell migration; cartilage development; neural tube closure; epithelial to mesenchymal transition; hindgut morphogenesis; positive regulation of inflammatory response; lung development. Disease: Robinow Syndrome, Autosomal Dominant

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800