ELISA/assay

Human Glutamate carboxypeptidase 2, FOLH1 ELISA Kit

MBS901525

48-Strip-Wells

510 USD

ELISA Kit

Human Glutamate carboxypeptidase 2, FOLH1 ELISA Kit

folate hydrolase (prostate-specific membrane antigen) 1

Human Glutamate carboxypeptidase 2 (FOLH1) ELISA kit; FGCP; FOLH; GCP2; GCPII; NAALAD1; NAALAdase; PSM; PSMA; mGCP; N-acetylated alpha-linked acidic dipeptidase 1; cell growth-inhibiting protein 27; folate hydrolase 1; folylpoly-gamma-glutamate carboxypep; folate hydrolase (prostate-specific membrane antigen) 1

glutamate carboxypeptidase 2 isoform 2; Glutamate carboxypeptidase 2; glutamate carboxypeptidase 2; NAALADase I; glutamate carboxylase II; glutamate carboxypeptidase II; membrane glutamate carboxypeptidase; cell growth-inhibiting gene 27 protein; folylpoly-gamma-glutamate carboxypeptidase; prostate specific membrane antigen variant F; pteroylpoly-gamma-glutamate carboxypeptidase; N-acetylated alpha-linked acidic dipeptidase 1; N-acetylated-alpha-linked acidic dipeptidase I; folate hydrolase (prostate-specific membrane antigen) 1; Cell growth-inhibiting gene 27 protein; Folate hydrolase 1; Folylpoly-gamma-glutamate carboxypeptidase; FGCP; Glutamate carboxypeptidase II; GCPII; Membrane glutamate carboxypeptidase; mGCP; N-acetylated-alpha-linked acidic dipeptidase I; NAALADase I; Prostate-specific membrane antigen; PSM; PSMA; Pteroylpoly-gamma-glutamate carboxypeptidase

FOLH1

FOLH1; FOLH1; PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase; FOLH; NAALAD1; PSM; PSMA; GIG27; FGCP; GCPII; mGCP; NAALADase I; PSM; PSMA

FOLH1_HUMAN

Human

719

This assay has high sensitivity and excellent specificity for detection of human FOLH1. No significant cross-reactivity or interference between human FOLH1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 4.7 ng/ml -300 ng/ml. Sensitivity: The minimum detectable dose of human FOLH1 is typically less than 1.2 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FOLH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FOLH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FOLH1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FOLH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

62548858

NP_001014986.1

NM_001014986.1

Q04609

84,331 Da

One Carbon Metabolism Pathway (198756); Vitamin Digestion And Absorption Pathway (199556); Vitamin Digestion And Absorption Pathway (199538)

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq, Jul 2010]

FOLH1: Has both folate hydrolase and N-acetylated-alpha-linked- acidic dipeptidase (NAALADase) activity. Has a preference for tri- alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N- aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression. Belongs to the peptidase M28 family. M28B subfamily. 8 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.17.21; Protease; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11p11.2. Cellular Component: membrane; integral to plasma membrane; cytoplasm. Molecular Function: dipeptidase activity; peptidase activity; metallopeptidase activity; carboxypeptidase activity; metal ion binding. Biological Process: folic acid and derivative metabolic process; proteolysis

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800