ELISA/assay

Mouse Melatonin receptor type 1A, MTNR1A ELISA Kit

MBS901510

48-Strip-Wells

510 USD

ELISA Kit

Mouse Melatonin receptor type 1A, MTNR1A ELISA Kit

melatonin receptor 1A

Mouse Melatonin receptor type 1A (MTNR1A) ELISA kit; MEL-1A-R; MT1; melatonin receptor type 1A; melatonin receptor 1A

melatonin receptor type 1A; Melatonin receptor type 1A; melatonin receptor type 1A; mel-1A-R; Mel1a receptor; melatonin receptor 1A

MTNR1A

Mtnr1a; Mtnr1a; MR; MelR; Mel-1A-R; Mel1a receptor

MTR1A_MOUSE

Mouse

353

This assay has high sensitivity and excellent specificity for detection of mouse MTNR1A. No significant cross-reactivity or interference between mouse MTNR1A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: The minimum detectable dose of mouse MTNR1A is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MTNR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MTNR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTNR1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTNR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

6678954

NP_032665.1

NM_008639.2

Q61184

39,837 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (575426); G Alpha (i) Signalling Events Pathway (575454); GPCR Downstream Signaling Pathway (575452); GPCR Ligand Binding Pathway (575425); GPCRs, Class A Rhodopsin-like Pathway (198308); Neuroactive Ligand-receptor Interaction Pathway (83250); Neuroactive Ligand-receptor Interaction Pathway (462); Signal Transduction Pathway (575318); Signaling By GPCR Pathway (575424); Small Ligand GPCRs Pathway (198310)

MTNR1A: High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family. Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; Motility/polarity/chemotaxis. Cellular Component: membrane; cell soma; integral to plasma membrane; integral to membrane; plasma membrane; receptor complex. Molecular Function: G-protein coupled receptor activity; signal transducer activity; hormone binding; melatonin receptor activity. Biological Process: circadian rhythm; G-protein coupled receptor protein signaling pathway; positive regulation of cGMP biosynthetic process; signal transduction; negative regulation of insulin secretion

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800