Human Triosephosphate isomerase, TPI1 ELISA Kit | MyBioSource MBS901437 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Triosephosphate isomerase, TPI1 ELISA Kit

catalog :

MBS901437

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS901437

products type :

ELISA Kit

products full name :

Human Triosephosphate isomerase, TPI1 ELISA Kit

products short name :

triosephosphate isomerase 1

products name syn :

Human Triosephosphate isomerase (TPI1) ELISA kit; MGC88108; TPI; ; triosephosphate isomerase 1

other names :

triosephosphate isomerase isoform 1; Triosephosphate isomerase; triosephosphate isomerase; triose-phosphate isomerase; epididymis secretory protein Li 49; triosephosphate isomerase 1; Triose-phosphate isomerase

products gene name :

TPI1

other gene names :

TPI1; TPI1; TIM; TPI; TPID; HEL-S-49; TPI; TIM

uniprot entry name :

TPIS_HUMAN

reactivity :

Human

sequence length :

249

specificity :

This assay has high sensitivity and excellent specificity for detection of human TPI1. No significant cross-reactivity or interference between human TPI1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 28 pg/ml -1800 pg/ml. Sensitivity: 7 pg /ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPI1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4507645

ncbi acc num :

NP_000356.1

ncbi gb acc num :

NM_000365.5

uniprot acc num :

P60174

ncbi mol weight :

30,791 Da

ncbi pathways :

Fatty Acid Beta Oxidation Pathway (198865); Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Gluconeogenesis Pathway (106204); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196); Glucose Metabolism Pathway (106199); Glycolysis Pathway (105911); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (413340); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (468194)

ncbi summary :

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

uniprot summary :

TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter. Protein type: Carbohydrate Metabolism - inositol phosphate; EC 5.3.1.1; Isomerase; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: extracellular space; cytosol; nucleus. Molecular Function: protein binding; triose-phosphate isomerase activity. Biological Process: pentose-phosphate shunt; glycolysis; multicellular organismal development; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis. Disease: Triosephosphate Isomerase Deficiency

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage