catalog number :
MBS901427
products type :
ELISA Kit
products full name :
Mouse Histone deacetylase complex subunit SAP130, SAP130 ELISA Kit
products short name :
Sin3A-associated protein, 130kDa
products name syn :
Mouse Histone deacetylase complex subunit SAP130 (SAP130) ELISA kit; FLJ12761; Sin3-associated polypeptide p130; histone deacetylase complex subunit SAP130; mSin3A-associated protein 130; Sin3A-associated protein; 130kDa
other names :
histone deacetylase complex subunit SAP130; Histone deacetylase complex subunit SAP130; histone deacetylase complex subunit SAP130; Sin3A associated protein, 130kDa; sin3-associated polypeptide p130; 130 kDa Sin3-associated polypeptide; Sin3A associated protein; 130 kDa Sin3-associated polypeptide; Sin3-associated polypeptide p130
products gene name :
SAP130
other gene names :
Sap130; Sap130; 6720406D06; 2610304F09Rik
uniprot entry name :
SP130_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse SAP130. No significant cross-reactivity or interference between mouse SAP130 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 46.88 pg/ml -3000 pg/ml. Sensitivity: 11.72 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SAP130 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SAP130 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SAP130 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SAP130 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_766553.1
ncbi gb acc num :
NM_172965.2
ncbi mol weight :
111,228 Da
uniprot summary :
SAP130: Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. Belongs to the SAP130 family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription factor; Acetyltransferase. Cellular Component: nucleoplasm; nucleus. Molecular Function: protein binding; histone acetyltransferase activity; transcription coactivator activity. Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
size4 :
10x96-Strip-Wells