product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAMTS5 ELISA Kit
catalog :
MBS901189
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS901189
products type :
ELISA Kit
products full name :
Mouse A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAMTS5 ELISA Kit
products short name :
ADAM metallopeptidase with thrombospondin type 1 motif, 5
products name syn :
Mouse A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA kit; ADAMTS11; ADMP-2; FLJ36738; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 5 (aggrecanase-2) ; aggrecanase-2; ADAM metallopeptidase with thrombospondin type 1 motif; 5
other names :
A disintegrin and metalloproteinase with thrombospondin motifs 5; A disintegrin and metalloproteinase with thrombospondin motifs 5; A disintegrin and metalloproteinase with thrombospondin motifs 5; ADMP-2; ADAMTS-5; ADAM-TS 5; implantin; aggrecanase-2; a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2); ADMP-2; Aggrecanase-2; Implantin
products gene name :
ADAMTS5
other gene names :
Adamts5; Adamts5; ASMP-2; ADAMTS1; ADAM-TS5; ADAMTS11; AI481094; 9530092O11Rik; ADAM-TS 5; ADAM-TS5; ADAMTS-5
uniprot entry name :
ATS5_MOUSE
reactivity :
Mouse
sequence length :
930
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse ADAMTS5. No significant cross-reactivity or interference between mouse ADAMTS5 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 40 ng/ml. Sensitivity: The minimum detectable dose of mouse ADAMTS5 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTS5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
125490361
ncbi acc num :
NP_035912.2
ncbi gb acc num :
NM_011782.2
uniprot acc num :
Q9R001
ncbi mol weight :
101,844 Da
ncbi pathways :
Endochondral Ossification Pathway (198336)
uniprot summary :
ADAMTS5: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period. Protein type: Secreted, signal peptide; Extracellular matrix; EC 3.4.24.-; Secreted; Motility/polarity/chemotaxis; Protease. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular region. Molecular Function: peptidase activity; metallopeptidase activity; zinc ion binding; hydrolase activity; metal ion binding; metalloendopeptidase activity. Biological Process: defense response to bacterium; proteolysis
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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