catalog number :
MBS901186
products type :
ELISA Kit
products full name :
Rat superoxide dismutase 1, soluble, SOD1 ELISA Kit
products short name :
superoxide dismutase 1, soluble
products name syn :
Rat superoxide dismutase 1; soluble (SOD1) ELISA kit; ALS; ALS1; IPOA; SOD; homodimer; Cu/Zn superoxide dismutase; SOD; soluble; indophenoloxidase A; superoxide dismutase; cystolic; superoxide dismutase 1; soluble
other names :
superoxide dismutase; Superoxide dismutase [Cu-Zn]; superoxide dismutase [Cu-Zn]; superoxide dismutase [Cu-Zn]; CuZn superoxide dismutase
products gene name :
SOD1
other gene names :
SOD1; SOD1
uniprot entry name :
SODC_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat SOD1. No significant cross-reactivity or interference between rat SOD1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: 3.9 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SOD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SOD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SOD1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SOD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001076096.1
ncbi gb acc num :
NM_001082627.1
ncbi mol weight :
15,819 Da
uniprot summary :
Function: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Catalytic activity: 2 superoxide + 2 H+ = O2 + H2O2. Cofactor: Binds 1 copper ion per subunit
size4 :
10x96-Strip-Wells