catalog number :
MBS901062
products type :
ELISA Kit
products full name :
Mouse Fibronectin type III domain-containing protein 5, FNDC5 ELISA Kit
products short name :
fibronectin type III domain containing 5
products name syn :
Mouse Fibronectin type III domain-containing protein 5 (FNDC5) ELISA kit; FRCP2; ; fibronectin type III domain containing 5
other names :
fibronectin type III domain-containing protein 5; Fibronectin type III domain-containing protein 5; fibronectin type III domain-containing protein 5; peroxisomal protein; fibronectin type III repeat-containing protein 2; fibronectin type III domain containing 5; Fibronectin type III repeat-containing protein 2; Peroxisomal protein; PeP
products gene name :
FNDC5
other gene names :
Fndc5; Fndc5; PeP; Pxp; C87088; AI836596; 1500001L03Rik; Frcp2; Pep; PeP
uniprot entry name :
FNDC5_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse FNDC5. No significant cross-reactivity or interference between mouse FNDC5 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml -400 pg/ml. Sensitivity: 1.56 pg /ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FNDC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FNDC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FNDC5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FNDC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_081678.1
ncbi gb acc num :
NM_027402.3
ncbi mol weight :
23,321 Da
uniprot summary :
FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Cellular Component: membrane; endoplasmic reticulum; extracellular region; integral to membrane; plasma membrane; peroxisome. Molecular Function: protein binding; hormone activity. Biological Process: response to muscle activity
size4 :
10x96-Strip-Wells