catalog number :
MBS901036
products type :
ELISA Kit
products full name :
Mouse Meteorin-like protein, METRNL ELISA Kit
products short name :
meteorin, glial cell differentiation regulator-like
products name syn :
Mouse Meteorin-like protein (METRNL) ELISA kit; MGC99788; ; meteorin; glial cell differentiation regulator-like
other names :
meteorin-like protein; Meteorin-like protein; meteorin-like protein; cometin; meteorin, glial cell differentiation regulator-like; Subfatin
products gene name :
METRNL
other gene names :
Metrnl; Metrnl; BC019776; 9430048M07Rik
uniprot entry name :
METRL_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse METRNL. No significant cross-reactivity or interference between mouse METRNL and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of mouse METRNL is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for METRNL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any METRNL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for METRNL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of METRNL bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_659046.1
ncbi gb acc num :
NM_144797.3
ncbi mol weight :
34,530 Da
uniprot summary :
METRNL: Belongs to the meteorin family. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; extracellular region. Molecular Function: hormone activity. Biological Process: fat cell differentiation; response to muscle activity; negative regulation of inflammatory response; brown fat cell differentiation; response to cold
size4 :
10x96-Strip-Wells