Human Zinc finger protein Aiolos, IKZF3 ELISA Kit | MyBioSource MBS900945 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Zinc finger protein Aiolos, IKZF3 ELISA Kit

catalog :

MBS900945

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS900945

products type :

ELISA Kit

products full name :

Human Zinc finger protein Aiolos, IKZF3 ELISA Kit

products short name :

IKAROS family zinc finger 3 (Aiolos)

products name syn :

Human Zinc finger protein Aiolos (IKZF3) ELISA kit; AIO; AIOLOS; ZNFN1A3; aiolos; zinc finger protein; subfamily 1A; 3 (Aiolos) ; IKAROS family zinc finger 3 (Aiolos)

other names :

zinc finger protein Aiolos isoform 7; Zinc finger protein Aiolos; zinc finger protein Aiolos; zinc finger DNA binding protein Aiolos; zinc finger protein, subfamily 1A, 3 (Aiolos); IKAROS family zinc finger 3 (Aiolos); Ikaros family zinc finger protein 3

products gene name :

IKZF3

other gene names :

IKZF3; IKZF3; AIO; AIOLOS; ZNFN1A3; ZNFN1A3

uniprot entry name :

IKZF3_HUMAN

reactivity :

Human

sequence length :

475

specificity :

This assay has high sensitivity and excellent specificity for detection of human IKZF3. No significant cross-reactivity or interference between human IKZF3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: The minimum detectable dose of human IKZF3 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IKZF3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IKZF3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IKZF3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IKZF3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

383872639

ncbi acc num :

NP_001244337.1

ncbi gb acc num :

NM_001257408.1

uniprot acc num :

Q9UKT9

ncbi mol weight :

58,023 Da

ncbi pathways :

IL2-mediated Signaling Events Pathway (137976)

ncbi summary :

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described, as well as some non-protein coding variants. [provided by RefSeq, Apr 2012]

uniprot summary :

Aiolos: a transcription factor of the ikaros C2H2-type zinc-finger protein family. Plays an important role in the regulation of lymphocyte differentiation. Deletions in Aiolos have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. 6 isoforms of the human protein are produced by alternative splicing. Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: cytoplasm; plasma membrane; nucleus. Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; sequence-specific DNA binding; metal ion binding; transcription factor activity. Biological Process: regulation of apoptosis; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; B cell activation; regulation of lymphocyte differentiation; regulation of B cell differentiation; regulation of B cell proliferation; mesoderm development

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage