catalog number :
MBS900924
products type :
ELISA Kit
products full name :
Mouse Hydroxyacid oxidase 1, HAO1 ELISA Kit
products short name :
hydroxyacid oxidase (glycolate oxidase) 1
products name syn :
Mouse Hydroxyacid oxidase 1 (HAO1) ELISA kit; GOX; GOX1; HAOX1; MGC142225; MGC142227; (S) -2-hydroxy-acid oxidase; glycolate oxidase; hydroxyacid oxidase 1; hydroxyacid oxidase (glycolate oxidase) 1
other names :
hydroxyacid oxidase 1; Hydroxyacid oxidase 1; hydroxyacid oxidase 1; HAOX1; glycolate oxidase 1; hydroxyacid oxidase 1, liver; Glycolate oxidase; GOX
products gene name :
HAO1
other gene names :
Hao1; Hao1; GOX; Gox1; Hao-1; Gox1; Hao-1; HAOX1; GOX
uniprot entry name :
HAOX1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse HAO1. No significant cross-reactivity or interference between mouse HAO1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 1.56 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAO1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_034533.1
ncbi gb acc num :
NM_010403.2
ncbi mol weight :
41,001 Da
ncbi pathways :
Glyoxylate And Dicarboxylate Metabolism Pathway (83200); Glyoxylate And Dicarboxylate Metabolism Pathway (383); Glyoxylate Metabolism Pathway (1001144); Metabolic Pathways (132962); Metabolism Pathway (971233); Metabolism Of Amino Acids And Derivatives Pathway (970519); Peroxisome Pathway (131230); Peroxisome Pathway (131126)
uniprot summary :
HAO1: Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2- hydroxy octanoate. Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Protein type: Oxidoreductase; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 1.1.3.15. Cellular Component: peroxisome. Molecular Function: glycolate oxidase activity; FMN binding; oxidoreductase activity; catalytic activity; receptor binding; (S)-2-hydroxy-acid oxidase activity. Biological Process: glycolate catabolic process; fatty acid alpha-oxidation
size4 :
10x96-Strip-Wells