ELISA/assay

Human Nucleoside diphosphate kinase A, NME1 ELISA Kit

MBS900914

48-Strip-Wells

510 USD

ELISA Kit

Human Nucleoside diphosphate kinase A, NME1 ELISA Kit

non-metastatic cells 1, protein (NM23A) expressed in

Human Nucleoside diphosphate kinase A (NME1) ELISA kit; AWD; GAAD; NB; NBS; NDPK-A; NDPKA; NM23; NM23-H1; NDP kinase A; OTTHUMP00000174772; non-metastatic cells 1; protein (NM23A) expressed in

nucleoside diphosphate kinase A isoform b; Nucleoside diphosphate kinase A; nucleoside diphosphate kinase A; NDP kinase A; granzyme A-activated DNase; metastasis inhibition factor nm23; tumor metastatic process-associated protein; non-metastatic cells 1, protein (NM23A) expressed in; NME/NM23 nucleoside diphosphate kinase 1; Granzyme A-activated DNase; GAAD; Metastasis inhibition factor nm23; NM23-H1; Tumor metastatic process-associated protein

NME1

NME1; NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1; NDPKA; NM23; NDK A; NDP kinase A; GAAD

NDKA_HUMAN

Human

152

This assay has high sensitivity and excellent specificity for detection of human NME1. No significant cross-reactivity or interference between human NME1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml -1200 pg/ml. Sensitivity: The minimum detectable dose of human NME1 is typically less than 4.68 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NME1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NME1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NME1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NME1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4557797

NP_000260.1

NM_000269.2

P15531

17,149 Da

Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (618580); Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (468242); Arf6 Downstream Pathway (138038); Arf6 Trafficking Events Pathway (137954); CMP Phosphorylation Pathway (908070); E-cadherin Signaling In The Nascent Adherens Junction Pathway (138005); Guanine Ribonucleotide Biosynthesis IMP = GDP,GTP Pathway (618581); Guanine Ribonucleotide Biosynthesis IMP = GDP,GTP Pathway (468243); Integrated Pancreatic Cancer Pathway (711360); Metabolic Pathways (132956)

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]

NME1: an enzyme with multiple activities including histidine protein kinase, nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase and 3 -5 exonuclease activities. Plays a major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Involved in cell proliferation, differentiation and development, G protein-coupled receptor endocytosis, cytotoxic T cell and NK cell-mediated cell death, and gene expression. Required for neural development including neural patterning and cell fate determination. During granzyme A (GZMA)-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3 end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Three alternatively spliced human proteins have been reported. Protein type: Nucleotide Metabolism - pyrimidine; Tumor suppressor; Nucleotide Metabolism - purine; Kinase, nucleoside diphosphate; EC 2.7.4.6; Kinase, other; Protein kinase, histidine; Other group; NDK family. Chromosomal Location of Human Ortholog: 17q21.3. Cellular Component: mitochondrion; membrane; cytoplasm; nucleus; cytosol. Molecular Function: identical protein binding; protein binding; GTP binding; nucleoside diphosphate kinase activity; magnesium ion binding; deoxyribonuclease activity; ATP binding; ribosomal small subunit binding. Biological Process: GTP biosynthetic process; nervous system development; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; UTP biosynthetic process; nucleoside diphosphate phosphorylation; endocytosis; DNA catabolic process; regulation of apoptosis; negative regulation of cell proliferation; CTP biosynthetic process; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; cell differentiation; positive regulation of epithelial cell proliferation; purine nucleotide metabolic process; positive regulation of DNA binding. Disease: Neuroblastoma, Susceptibility To

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800