Human Protein Wnt-16, WNT16 ELISA Kit | MyBioSource MBS900911 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Protein Wnt-16, WNT16 ELISA Kit

catalog :

MBS900911

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS900911

products type :

ELISA Kit

products full name :

Human Protein Wnt-16, WNT16 ELISA Kit

products short name :

wingless-type MMTV integration site family, member 16

products name syn :

Human Protein Wnt-16 (WNT16) ELISA kit; wingless-type MMTV integration site family; member 16

other names :

protein Wnt-16 isoform 2; Protein Wnt-16; protein Wnt-16; wingless-type MMTV integration site family member 16b; wingless-type MMTV integration site family, member 16

products gene name :

WNT16

other gene names :

WNT16; WNT16

uniprot entry name :

WNT16_HUMAN

reactivity :

Human

sequence length :

355

specificity :

This assay has high sensitivity and excellent specificity for detection of human WNT16. No significant cross-reactivity or interference between human WNT16 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: The minimum detectable dose of human WNT16 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT16 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT16 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

17402914

ncbi acc num :

NP_057171.2

ncbi gb acc num :

NM_016087.2

uniprot acc num :

Q9UBV4

ncbi mol weight :

40,690 Da

ncbi pathways :

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Class B/2 (Secretin Family Receptors) Pathway (106378); DNA Damage Response (only ATM Dependent) Pathway (198827); Disease Pathway (530764); GPCR Ligand Binding Pathway (161020); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83063); Hedgehog Signaling Pathway (474)

ncbi summary :

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It contains two transcript variants diverging at the 5' termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. They are differentially expressed in normal tissues, one of which (variant 2) is expressed at significant levels only in the pancreas, whereas another one (variant 1) is expressed more ubiquitously with highest levels in adult kidney, placenta, brain, heart, and spleen. [provided by RefSeq, Jul 2008]

uniprot summary :

WNT16: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 7q31. Cellular Component: proteinaceous extracellular matrix; extracellular space; cytoplasm; extracellular region. Molecular Function: frizzled binding. Biological Process: neuron differentiation; positive regulation of phosphoinositide 3-kinase cascade; keratinocyte differentiation; Wnt receptor signaling pathway; cell fate commitment; positive regulation of JNK cascade; keratinocyte proliferation; bone remodeling

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage