Human PDZ and LIM domain protein 5, PDLIM5 ELISA Kit | MyBioSource MBS900863 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PDZ and LIM domain protein 5, PDLIM5 ELISA Kit

catalog :

MBS900863

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS900863

products type :

ELISA Kit

products full name :

Human PDZ and LIM domain protein 5, PDLIM5 ELISA Kit

products short name :

PDZ and LIM domain 5

products name syn :

Human PDZ and LIM domain protein 5 (PDLIM5) ELISA kit; ENH; ENH1; L9; LIM; enigma homolog; enigma-like LIM domain protein; PDZ and LIM domain 5

other names :

PDZ and LIM domain protein 5 isoform d; PDZ and LIM domain protein 5; PDZ and LIM domain protein 5; enigma homolog; enigma-like LIM domain protein; enigma-like PDZ and LIM domains protein; PDZ and LIM domain 5; Enigma homolog; Enigma-like PDZ and LIM domains protein

products gene name :

PDLIM5

other gene names :

PDLIM5; PDLIM5; L9; ENH; LIM; ENH1; ENH; L9

uniprot entry name :

PDLI5_HUMAN

reactivity :

Human

sequence length :

234

specificity :

This assay has high sensitivity and excellent specificity for detection of human PDLIM5. No significant cross-reactivity or interference between human PDLIM5 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml -1200 pg/ml. Sensitivity: The minimum detectable dose of human PDLIM5 is typically less than 4.68 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDLIM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDLIM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDLIM5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDLIM5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

58533159

ncbi acc num :

NP_001011515.1

ncbi gb acc num :

NM_001011515.2

uniprot acc num :

Q96HC4

ncbi mol weight :

63,945 Da

ncbi summary :

This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]

uniprot summary :

LIM: an adaptor protein of the Enigma family. Contains three LIM domains and one PDZ domain. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. Enigma family proteins possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 4q22. Cellular Component: postsynaptic membrane; neuron projection; membrane; postsynaptic density; cytosol; Z disc; cell junction; actin cytoskeleton. Molecular Function: actinin binding; protein kinase C binding; zinc ion binding; protein N-terminus binding; actin binding. Biological Process: regulation of synaptogenesis

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage