Human Endophilin-A2, SH3GL1 ELISA Kit | MyBioSource MBS900764 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Endophilin-A2, SH3GL1 ELISA Kit

catalog :

MBS900764

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS900764

products type :

ELISA Kit

products full name :

Human Endophilin-A2, SH3GL1 ELISA Kit

products short name :

SH3-domain GRB2-like 1

products name syn :

Human Endophilin-A2 (SH3GL1) ELISA kit; CNSA1; EEN; MGC111371; SH3D2B; SH3P8; SH3 domain GRB2-like 1; SH3-containing Grb-2-like 1 protein; SH3-containing protein EEN; extra 11-19 leukemia fusion; fusion partner of MLL; SH3-domain GRB2-like 1

other names :

endophilin-A2 isoform 2; Endophilin-A2; endophilin-A2; endophilin-2; SH3 domain protein 2B; EEN fusion partner of MLL; extra 11-19 leukemia fusion; SH3-containing Grb-2-like 1 protein; SH3 domain-containing GRB2-like protein 1; extra eleven-nineteen leukemia fusion gene protein; SH3-domain GRB2-like 1; EEN fusion partner of MLL; Endophilin-2; Extra eleven-nineteen leukemia fusion gene protein; EEN; SH3 domain protein 2B; SH3 domain-containing GRB2-like protein 1

products gene name :

SH3GL1

other gene names :

SH3GL1; SH3GL1; EEN; CNSA1; SH3P8; SH3D2B; CNSA1; SH3D2B; EEN

uniprot entry name :

SH3G1_HUMAN

reactivity :

Human

sequence length :

320

specificity :

This assay has high sensitivity and excellent specificity for detection of human SH3GL1. No significant cross-reactivity or interference between human SH3GL1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: The minimum detectable dose of human SH3GL1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SH3GL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SH3GL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SH3GL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SH3GL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

317108191

ncbi acc num :

NP_001186872.1

ncbi gb acc num :

NM_001199943.1

uniprot acc num :

Q99961

ncbi mol weight :

41,490 Da

ncbi pathways :

Endocytosis Pathway (102279); Endocytosis Pathway (102181); Signaling Events Mediated By Focal Adhesion Kinase Pathway (169348)

ncbi summary :

This gene encodes a member of the endophilin family of Src homology 3 domain-containing proteins. The encoded protein is involved in endocytosis and may also play a role in the cell cycle. Overexpression of this gene may play a role in leukemogenesis, and the encoded protein has been implicated in acute myeloid leukemia as a fusion partner of the myeloid-lymphoid leukemia protein. Pseudogenes of this gene are located on the long arm of chromosomes 11 and 17. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

uniprot summary :

SH3GL1: Implicated in endocytosis. May recruit other proteins to membranes with high curvature. In some cases of acute leukemia, a translocation results in the formation of a MLL-EEN fusion gene. Belongs to the endophilin family. Protein type: Oncoprotein; Vesicle. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: cell projection; early endosome membrane; cytoplasm; podosome; cell junction. Molecular Function: identical protein binding; protein binding; GTPase binding; lipid binding; phosphatase binding. Biological Process: central nervous system development; endocytosis; signal transduction. Disease: Leukemia, Acute Myeloid

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage