Mouse Cathepsin L1, CTSL1 ELISA Kit | MyBioSource MBS900575 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Cathepsin L1, CTSL1 ELISA Kit

catalog :

MBS900575

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS900575

products type :

ELISA Kit

products full name :

Mouse Cathepsin L1, CTSL1 ELISA Kit

products short name :

cathepsin L1

products name syn :

Mouse Cathepsin L1 (CTSL1) ELISA kit; CATL; CTSL; FLJ31037; MEP; cathepsin L; major excreted protein; cathepsin L1

other names :

cathepsin L1 preproprotein; Cathepsin L1; cathepsin L1; Cat L; nackt; furless; major excreted protein; p39 cysteine proteinase; cathepsin L; Cathepsin L; Major excreted protein; MEP; p39 cysteine proteinaseCleaved into the following 2 chains:Cathepsin L1 heavy chain; Cathepsin L1 light chain

products gene name :

CTSL1

other gene names :

Ctsl; Ctsl; fs; MEP; nkt; Ctsl1; 1190035F06Rik; Ctsl1; MEP

uniprot entry name :

CATL1_MOUSE

reactivity :

Mouse

sequence length :

334

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse CTSL1. No significant cross-reactivity or interference between mouse CTSL1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: The minimum detectable dose of mouse CTSL1 is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CTSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTSL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6753558

ncbi acc num :

NP_034114.1

ncbi gb acc num :

NM_009984.3

uniprot acc num :

P06797

ncbi mol weight :

37,547 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (1001029); Adaptive Immune System Pathway (971210); Antigen Processing And Presentation Pathway (83271); Antigen Processing And Presentation Pathway (485); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1001018); Collagen Degradation Pathway (1001030); Collagen Formation Pathway (1001016); Degradation Of The Extracellular Matrix Pathway (1001028); Endochondral Ossification Pathway (198336); Extracellular Matrix Organization Pathway (1001015)

ncbi summary :

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the activation peptide and the cathepsin L1 heavy and light chains. The mature enzyme appears to be important in embryonic development through its processing of histone H3 and may play a role in disease progression in a model of kidney disease. Homozygous knockout mice for this gene exhibit hair loss, skin thickening, bone and heart defects, and enhanced susceptibility to bacterial infection. A pseudogene of this gene has been identified in the genome. [provided by RefSeq, Aug 2015]

uniprot summary :

CTSL2: Cysteine protease. May have an important role in corneal physiology. Belongs to the peptidase C1 family. Protein type: Secreted, signal peptide; Protease; Secreted; EC 3.4.22.15. Cellular Component: extracellular space; neuron projection; microvillus; lysosome; apical part of cell; cytoplasm; nucleolus; vacuole; perikaryon; cytoplasmic vesicle; nucleus; external side of plasma membrane; secretory granule. Molecular Function: peptidase activity; protein binding; hydrolase activity; histone binding; cysteine-type endopeptidase activity; protein complex binding; kininogen binding; cysteine-type carboxypeptidase activity; aminopeptidase activity; peptide binding; cysteine-type peptidase activity. Biological Process: protein autoprocessing; catagen; hair follicle morphogenesis; cell communication; protein processing; decidualization; proteolysis

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage