catalog number :
MBS900440
products type :
ELISA Kit
products full name :
Bovine Transcobalamin-2, TCN2 ELISA Kit
products short name :
transcobalamin II; macrocytic anemia
products name syn :
Bovine Transcobalamin-2 (TCN2) ELISA kit; D22S676; D22S750; TC2; transcobalamin II; transcobalamin II; macrocytic anemia
other names :
transcobalamin-2; Transcobalamin-2; transcobalamin-2; TC-2; TCII; TC II; transcobalamin II; macrocytic anemia; transcobalamin II<; Transcobalamin II; TC II; TCII
products gene name :
TCN2
other gene names :
TCN2; TCN2; TC-2; TC II; TCII
uniprot entry name :
TCO2_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine TCN2. No significant cross-reactivity or interference between Bovine TCN2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm
other info2 :
Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 2: Anion transport. Protein Biological Process 3: Cobalt transport
products description :
For the quantitative determination of bovine transcobalamin-2 (TCN2) concentrations in serum, plasma, tissue homogenates. PRINCIPLE This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TCN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TCN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TCN2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TCN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_776620.2
ncbi gb acc num :
NM_174195.3
ncbi mol weight :
47,958 Da
ncbi pathways :
Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway 942901!!Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway 942932!!Defective BTD Causes Biotidinase Deficiency Pathway 942802!!Defective CD320 Causes Methylmalonic Aciduria Pathway 942803!!Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway 942931!!Defective GIF Causes Intrinsic Factor Deficiency Pathway 942935!!Defective HLCS Causes Multiple Carboxylase Deficiency Pathway 942832!!Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway 942933!!Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway 942920!!Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway 942923
uniprot summary :
Function: Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells.