ELISA/assay

Human Alpha-amylase 2B, AMY2B ELISA Kit

MBS900351

48-Strip-Wells

510 USD

ELISA Kit

Human Alpha-amylase 2B, AMY2B ELISA Kit

amylase, alpha 2B (pancreatic)

Human Alpha-amylase 2B (AMY2B) ELISA kit; AMY2; 1; 4-alpha-D-glucan glucanohydrolase; alpha-amylase carcinoid; amylase; pancreatic; alpha-2B; glycogenase; amylase; alpha 2B (pancreatic)

alpha-amylase 2B; Alpha-amylase 2B; alpha-amylase 2B; glycogenase; carcinoid alpha-amylase; 1,4-alpha-D-glucan glucanohydrolase 2B; amylase, alpha 2B (pancreatic); 1,4-alpha-D-glucan glucanohydrolase 2B; Carcinoid alpha-amylase

AMY2B

AMY2B; AMY2B; HXA; AMY2; AMY3

AMY2B_HUMAN

Human

511

This assay has high sensitivity and excellent specificity for detection of human AMY2B. No significant cross-reactivity or interference between human AMY2B and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 39.06 mU /ml - 2500 mU /ml. Sensitivity: The minimum detectable dose of human AMY2B is typically less than 9.77 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AMY2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AMY2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMY2B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMY2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

10280622

NP_066188.1

NM_020978.4

P19961

57,710 Da

Carbohydrate Digestion And Absorption Pathway (170720); Carbohydrate Digestion And Absorption Pathway (170654); Digestion Of Dietary Carbohydrate Pathway (106197); Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Carbohydrates Pathway (106196); Myoclonic Epilepsy Of Lafora Pathway (980469); Pancreatic Secretion Pathway (169306)

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jun 2013]

AMY2B: Monomer. Belongs to the glycosyl hydrolase 13 family. Protein type: Hydrolase; Carbohydrate Metabolism - starch and sucrose; Secreted, signal peptide; EC 3.2.1.1; Secreted. Chromosomal Location of Human Ortholog: 1p21. Molecular Function: alpha-amylase activity; metal ion binding. Biological Process: carbohydrate metabolic process; digestion

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800