ELISA/assay

Mouse Tyrosine-protein kinase JAK3, JAK3 ELISA Kit

MBS900324

48-Strip-Wells

510 USD

ELISA Kit

Mouse Tyrosine-protein kinase JAK3, JAK3 ELISA Kit

Janus kinase 3 (a protein tyrosine kinase, leukocyte)

Mouse Tyrosine-protein kinase JAK3 (JAK3) ELISA kit; JAK-3; JAK3_HUMAN; JAKL; L-JAK; LJAK; Janus kinase 3 (a protein tyrosine kinase; leukocyte) ; leukocyte Janus kinase; tyrosine-protein kinase JAK3; Janus kinase 3 (a protein tyrosine kinase; leukocyte)

tyrosine-protein kinase JAK3; Tyrosine-protein kinase JAK3; tyrosine-protein kinase JAK3; Janus kinase 3; Janus kinase 3; JAK-3

JAK3

Jak3; Jak3; fae; wil; JAK-3

JAK3_MOUSE

Mouse

1100

This assay has high sensitivity and excellent specificity for detection of Mouse JAK3. No significant cross-reactivity or interference between Mouse JAK3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Adaptive immunity

For samples: For the quantitative determination of mouse tyrosine-protein kinase JAK3 (JAK3) concentrations in serum, plasma, tissue homogenates, cell lysates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for JAK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any JAK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for JAK3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of JAK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

300192999

NP_001177759.1

NM_001190830.1

Q62137

122,641 Da

Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Cytokine Signaling In Immune System Pathway (970814); Epstein-Barr Virus Infection Pathway (585576); Epstein-Barr Virus Infection Pathway (587115); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); IL-2 Signaling Pathway (198331); IL-4 Signaling Pathway (198315)

JAK3: a non-receptor tyrosine-kinase of the Jak family expressed predominantly in immune cells. Mediates signal transduction via the common gamma-chain of cytokine receptors, including IL-2, -4, -7, -9, and -15. Interacts with members of the STAT (signal transduction and activators of transcription) family. Interacts with STAM2 and SHB. Contains two protein kinase domains. Mutations that abrogate JAK3 function cause an autosomal SCID (severe combined immunodeficiency disease). Inhibitor: R017s for organ transplants. Three differentially spliced isoforms have been described. Protein type: Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; JakA family. Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; membrane; cytoplasm; intracellular; cytosol. Molecular Function: transferase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; protein phosphatase binding; ATP binding; protein kinase activity; hormone receptor binding. Biological Process: positive regulation of immune response; peptidyl-tyrosine phosphorylation; negative regulation of interleukin-10 production; negative regulation of FasL biosynthetic process; negative regulation of T-helper 1 cell differentiation; protein amino acid autophosphorylation; positive regulation of activated T cell proliferation; enzyme linked receptor protein signaling pathway; protein amino acid phosphorylation; elevation of cytosolic calcium ion concentration; erythrocyte differentiation; T cell homeostasis; positive regulation of T cell proliferation; inflammatory response; negative regulation of interleukin-12 production; cell migration; immune system process; cytokine and chemokine mediated signaling pathway; lymph node development; tyrosine phosphorylation of Stat5 protein; negative regulation of dendritic cell cytokine production; tyrosine phosphorylation of STAT protein; B cell differentiation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of calcium ion transport; negative regulation of T cell activation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800