Human A disintegrin and metalloproteinase with thrombospondin motifs 12, ADAMTS12 ELISA Kit | MyBioSource MBS900261 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human A disintegrin and metalloproteinase with thrombospondin motifs 12, ADAMTS12 ELISA Kit

catalog :

MBS900261

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS900261

products type :

ELISA Kit

products full name :

Human A disintegrin and metalloproteinase with thrombospondin motifs 12, ADAMTS12 ELISA Kit

products short name :

ADAM metallopeptidase with thrombospondin type 1 motif, 12

products name syn :

Human A disintegrin and metalloproteinase with thrombospondin motifs 12 (ADAMTS12) ELISA kit; UNQ1918; PRO4389; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 12; ADAM metallopeptidase with thrombospondin type 1 motif; 12

other names :

A disintegrin and metalloproteinase with thrombospondin motifs 12 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 12; A disintegrin and metalloproteinase with thrombospondin motifs 12; ADAM-TS12; ADAMTS-12; ADAM-TS 12; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 12; ADAM metallopeptidase with thrombospondin type 1 motif, 12

products gene name :

ADAMTS12

other gene names :

ADAMTS12; ADAMTS12; PRO4389; UNQ1918/PRO4389; ADAM-TS 12; ADAM-TS12; ADAMTS-12

uniprot entry name :

ATS12_HUMAN

reactivity :

Human

sequence length :

1594

specificity :

This assay has high sensitivity and excellent specificity for detection of Human ADAMTS12. No significant cross-reactivity or interference between Human ADAMTS12 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 47 pg/ml-3000 pg/ml. Sensitivity: 11.75 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTS12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

51558724

ncbi acc num :

NP_112217.2

ncbi gb acc num :

NM_030955.2

uniprot acc num :

P58397

ncbi mol weight :

177,676 Da

ncbi pathways :

Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (980460); O-linked Glycosylation Pathway (980459); Post-translational Protein Modification Pathway (161012)

ncbi summary :

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS-1) motif. Individual members of this family differ in the number of C-terminal TS-1 motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains eight TS-1 motifs. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. [provided by RefSeq, Jul 2008]

uniprot summary :

ADAMTS12: Metalloprotease that may play a role in the degradation of COMP. Cleaves also alpha-2 macroglobulin and aggregan. Has anti-tumorigenic properties. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted; EC 3.4.24.-; Protease; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 5q35. Cellular Component: extracellular matrix; proteinaceous extracellular matrix. Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity. Biological Process: cell migration; proteolysis involved in cellular protein catabolic process; cell-matrix adhesion; negative regulation of chondrocyte differentiation; proteoglycan catabolic process; regulation of inflammatory response

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage