catalog number :
MBS900227
products type :
ELISA Kit
products full name :
Mouse serum amyloid A1, SAA1 ELISA Kit
products short name :
serum amyloid A1
products name syn :
Mouse serum amyloid A1 (SAA1) ELISA kit; MGC111216; PIG4; SAA; TP53I4; tumor protein p53 inducible protein 4; serum amyloid A1
other names :
serum amyloid A-1 protein; Serum amyloid A-1 protein; serum amyloid A-1 protein; serum amyloid A 2; serum amyloid A 1
products gene name :
SAA1
other gene names :
Saa1; Saa1; Saa2; Saa-1
uniprot entry name :
SAA1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse SAA1. No significant cross-reactivity or interference between mouse SAA1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: 0.078 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SAA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SAA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SAA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SAA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_033143.1
ncbi gb acc num :
NM_009117.3
ncbi mol weight :
13,770 Da
ncbi pathways :
Activated TLR4 Signalling Pathway (970703); Advanced Glycosylation Endproduct Receptor Signaling Pathway (1001253); Amyloids Pathway (1000612); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (1000591); Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1001263); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (1001271); Disease Pathway (970606); Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway (1000948); G Alpha (i) Signalling Events Pathway (1000973)
uniprot summary :
SAA1: Major acute phase reactant. Apolipoprotein of the HDL complex. Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA1 protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Elevated serum SAA1 protein levels may be associated with lung cancer. Belongs to the SAA family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space; extracellular region. Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; chemoattractant activity. Biological Process: cholesterol metabolic process; acute-phase response
size4 :
10x96-Strip-Wells